Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: gastritis [ICD11:
DA42 
];
gastric ulcer [ICD11:
DA60 , ICD11:
XN3DY ];
cancer [ICD11:
2D4Z ];
infection due to Helicobacter pylori [ICD11:
XN3DY ]
The structure was elucidated in this paperNCBI PubMed ID: 19889074Journal NLM ID: 9605411Publisher: Blackwell Science
Correspondence: mac
ua.pt
Institutions: Departamento de Quimica da Universidade de Aveiro, Campus de Santiago, Aveiro, Portugal
BACKGROUND: Helicobacter pylori cell surface is composed of lipopolysaccharides (LPSs) yielding structures homologous to mammalian Lewis O-chains blood group antigens. These structures are key mediators in the definition of host-microbial interactions and known to change their expression pattern in response to environmental pressure. AIMS: The present work is focused on the identification of new H. pylori cell-surface glycosides. Special attention is further devoted to provide insights on the impact of in vitro subcultivation on H. pylori cell-surface phenotypes. METHODS: Cell-surface glycans from H. pylori NCTC 11637 and two clinical isolates were recovered from the aqueous phase resulting from phenol:water extraction of intact bacteria. They were evaluated in relation to their sugars and glycosidic-linkages composition by CG-MS, size-exclusion chromatography, NMR, and Mass Spectrometry. H. pylori glycan profile was also monitored during subcultivation in vitro in agar and F12 liquid medium. RESULTS: All three studied strains produce LPS expressing Lewis epitopes and express bioaccumulate amylose-like glycans. Bioaccumulation of amylose was found to be enhanced with the subcultivation of the bacterium on agar medium and accompanied by a decrease in the expression of LPS O-chains. In contrast, during exponential growth in F12 liquid medium, an opposite behavior is observed, that is, there is an increase in the overall amount of LPS and decrease in amylose content. CONCLUSIONS: This work shows that under specific environmental conditions, H. pylori expresses a phase-variable cell-surface α-(1→4)-glucose moiety
Helicobacter pylori, glucan, cell-surface polysaccharide, environmental pressure
Structure type: homopolymer
Location inside paper: p.568
Trivial name: methyl glucose lipopolysaccharide, glucan, maltosaccharide, α-1,4-D-glucan, amylose, α-glucan, glycogen backbone, α-(1,4)-glucan, starch, α-(1-4)-glucan, starch, glycogen
Compound class: CPS, EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, methyl glucose lipopolysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 1H NMR, methylation, GC-MS, ESI-MS, composition analysis, CID-MS/MS, statistical analysis
Related record ID(s): 913, 8387, 10122, 22727, 23696, 102751, 106369
NCBI Taxonomy refs (TaxIDs): 210Reference(s) to other database(s): GTC:G05740LL, GlycomeDB:
12100, CCSD:
4943, CBank-STR:819
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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