Ganguly J, Choudhury BP, Balakrish Nair G, Sen AK Structural characterization of the O-antigenic polysaccharide from the lipopolysaccharide of Vibrio cholerae O37 Indian Journal of Chemistry48(5) (2009)
729-734
The chemical structure of the O-antigenic polysaccharide isolated from the lipopolysaccharide of Vibrio cholerae O37 by mild acid hydrolysis was elucidated. The O-antigenic polysaccharide is found to consist of D-glucose, N-acetyl-D-Quinovosamine and small amount of 4-O-methyl-N-acetyl-D-quinovasamine. The structure of the O-antigen is established by using sugar and methylation analyses, Smith degradation studies and by using GLC, GC-MS, FAB-MS, one dimensional 1H and 13C NMR spectroscopy and two dimensional NMR spectroscopy including COSY, TOCSY, HSQC, experiments.
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, FAB-MS, GC-MS, SDS-PAGE, sugar analysis, acid hydrolysis, GLC, Smith degradation Comments, role: oligosaccharides A and B (formed via intramolecular rearrangent) obtained from the Smith degraded OPS.
Found 
report error
rediffmail.com; arsen