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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: gastroenteritis [ICD11: 1A40.0

]; bacteremia [ICD11: MA15.0

]; septicemia [ICD11: MA15.Y

]

NCBI PubMed ID: 19151135
Publication DOI: 10.1128/JB.01395-08
Journal NLM ID: 2985120R
Publisher: American Society for Microbiology
Correspondence: jtomas

ub.edu
Institutions: Departamento de Microbiologia, Facultad de Biologia, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain, Departamento de Microbiologia y Parasitologia Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain

Comparison between the lipopolysaccharide (LPS) core structures of Aeromonas salmonicida subsp. salmonicida A450 and Aeromonas hydrophila AH-3 shows great similarity in the inner LPS core and part of the outer LPS core but some differences in the distal part of the outer LPS core (residues ld-Hep, d-Gal, and d-GalNAc). The three genomic regions encoding LPS core biosynthetic genes in A. salmonicida A450, of which regions 2 and 3 have genes identical to those of A. hydrophila AH-3, were fully sequenced. A. salmonicida A450 region 1 showed seven genes: three identical to those of A. hydrophila AH-3, three similar but not identical to those of A. hydrophila AH-3, and one without any homology to any well-characterized gene. A. salmonicida A450 mutants with alterations in the genes that were not identical to those of A. hydrophila AH-3 were constructed, and their LPS core structures were fully elucidated. At the same time, all the A. salmonicida A450 genes identical to those of A. hydrophila AH-3 were used to complement the previously obtained A. hydrophila AH-3 mutants for each of these genes. Combining the gene sequence and complementation test data with the structural data and phenotypic characterization of the mutant LPSs enabled a presumptive assignment of all LPS core biosynthesis gene functions in A. salmonicida A450. Furthermore, hybridization studies with internal probes for the A. salmonicida-specific genes using different A. salmonicida strains (strains of different subspecies or atypical strains) showed a unique or prevalent LPS core type, which is the one fully characterized for A. salmonicida A450.

biosynthesis, lipopolysaccharide core, Aeromonas salmonicida

Structure type: oligomer
Location inside paper: p.2229, fig.1B
Aglycon: (2->?)lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_130650,IEDB_136044,IEDB_137472,IEDB_137777,IEDB_137779,IEDB_138949,IEDB_139428,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_2189046,IEDB_2189047,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88

Methods: methylation, SDS-PAGE, DNA techniques, MALDI-TOF MS, genetic methods
Biosynthesis and genetic data: genetic data
Comments, role: O-antigen is attached to Gal residue [ref. 16]

Related record ID(s): 23724, 24014
NCBI Taxonomy refs (TaxIDs): 600645
Reference(s) to other database(s): GTC:G10509UC, GlycomeDB:27455
Show glycosyltransferases

There is only one chemically distinct structure:

N[C@H]1[C@@H](OC[C@H](O)[C@H]2O[C@H](O[C@H]3[C@@H](O)[C@H](O)[C@@H]([C@@H](O)CO)O[C@@H]3O[C@@H]3[C@H](O)[C@@H](O[C@@H]4[C@H](OP(=O)(O)O)C[C@](O)(C(=O)O)O[C@@H]4[C@H](O)CO)O[C@H]([C@H](CO)O[C@H]4O[C@H]([C@@H](O)CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@H]3O[C@@H]3O[C@H](CO[C@H]4O[C@H]([C@@H](CO)O[C@H]5O[C@H]([C@H](O)CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@@H](O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@@H]4O)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@@H](O)[C@@H]2O)O[C@H](CO)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize