Taxonomic group: bacteria / Chlamydiae
(Phylum: Chlamydiae)
Associated disease: infection due to Chlamydia trachomatis [ICD11:
XN4Q4 
]
The structure was elucidated in this paperNCBI PubMed ID: 12542694Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: hbrade
fz-borstel.de
Institutions: Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany
The lipopolysaccharide (LPS) of Chlamydia trachomatis serotype E was isolated from tissue culture-grown elementary bodies and analyzed structurally by mass spectrometry and 1H, 13C and 31P nuclear magnetic resonance. The LPS is composed of the same pentasaccharide bisphosphate αKdo-(2-8)-αKdo-(2-4)-αKdo-(2-6)-βGlcN-4P-(1-6) αGlcN-1P (Kdo is 3-deoxy-α-D-manno-oct-2-ulosonic acid) as reported for C. trachomatis serotype L2[Rund, S., Lindner, B., Brade, H. and Holst, O. (1999) J. Biol. Chem. 274, 16819-16824]. The glucosamine disaccharide backbone is substituted with a complex mixture of fatty acids with ester or amide linkage whereby no ester-linked hydroxy fatty acids were found. The LPS was purified carefully (with contaminations by protein or nucleic acids below 0.3%) and tested for its ability to induce proinflammatory cytokines in several readout systems in comparison to LPS from C. trachomatis serotype L2 and Chlamydophila psittaci strain 6BC as well as enterobacterial smooth and rough LPS and synthetic hexaacyl lipid A. The chlamydial LPS were at least 10 times less active than typical endotoxins; specificity of the activities was confirmed by inhibition with the LPS antagonist, B1233, or with monoclonal antibodies against chlamydial LPS. Like other LPS, the chlamydial LPS used toll-like receptor TLR4 for signalling, but unlike other LPS activation was strictly CD14-dependent.
LPS, Chlamydia, Chlamydia trachomatis, Chlamydophila psittaci, MALDI-TOF MS, toll-like receptors, innate immunity, ESI-FTIR-MS, ESI-FT-IR MS
Structure type: oligomer
Location inside paper: p.444
Trivial name: core-lipid A carbohydrate backbone, epitope G
Compound class: core oligosaccharide, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130657,IEDB_130658,IEDB_130659,IEDB_135394,IEDB_141807,IEDB_150760,IEDB_150908,IEDB_151531
Methods: HPAEC
Biological activity: signalling, serological data, interaction with human MNC
Comments, role: minor component
Related record ID(s): 2501, 2540, 2541, 2542, 4382, 4383, 6418, 25194, 124181
NCBI Taxonomy refs (TaxIDs): 813Reference(s) to other database(s): GlycomeDB:
8180, CCSD:
46734, CBank-STR:16077
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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