Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Bos taurus; Homo sapiens
Associated disease: anthrax [ICD11:
1B97 
, ICD11:
XN94F ];
pneumonia [ICD11:
CA40 ];
infection due to Bacillus anthracis [ICD11:
XN94F ];
infection due to Bacillus cereus [ICD11:
XN8PY ]
NCBI PubMed ID: 19270075Publication DOI: 10.1093/glycob/cwp036Journal NLM ID: 9104124Publisher: IRL Press at Oxford University Press
Correspondence: rcarlson
ccrc.uga.edu
Institutions: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS D-11, Atlanta, GA 30333, USA, Departments of Microbiology and Biotechnology, University of Tübingen, D-72076 Tübingen, Germany
The immunoreactivities of hydrogen fluoride- (HF) released cell wall polysaccharides (HF-PSs) from selected Bacillus anthracis and Bacillus cereus strains were compared using antisera against live and killed B. anthracis spores. These antisera bound to the HF-PSs from B. anthracis and from three clinical B. cereus isolates (G9241, 03BB87 and 03BB102) obtained from cases of severe or fatal human pneumonia but did not bind the HF-PSs from the closely related B. cereus ATCC 10987 or from B. cereus type strain ATCC 14579. Antiserum against a keyhole limpet hemocyanin conjugate of the B. anthracis HF-PS (HF-PS-KLH) also bound HF-PSs and cell walls from B. anthracis and the three clinical B. cereus isolates, and B. anthracis spores. These results indicate that the B. anthracis HF-PS is an antigen in both B. anthracis cell walls and spores, and that it shares cross-reactive, and possibly pathogenicity-related, epitopes with three clinical B. cereus isolates that caused severe disease. The anti-HF-PS-KLH antiserum cross-reacted with the bovine serum albumin (BSA)-conjugates of all B. anthracis and all B. cereus HF-PSs tested, including those from non-clinical B. cereus ATCC 10987 and ATCC 14579 strains. Finally, the serum of vaccinated (anthrax vaccine adsorbed [AVA]) Rhesus macaques that survived inhalation anthrax contained IgG antibodies that bound the B. anthracis HF-PS-KLH conjugate. These data indicate that HF-PSs from the cell walls of the bacilli tested here are (i) antigens that contain (ii) a potentially virulence-associated carbohydrate antigen motif, and (iii) another antigenic determinant that is common to B. cereus strains.
antigens, specificity, polysaccharides, Bacillus anthracis, Bacillus cereus, polysaccharides specificity
Structure type: structural motif or average structure
Location inside paper: p.666, fig.1
Trivial name: HF-PS
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_141807,IEDB_151531,IEDB_423151,IEDB_885813,IEDB_885822
Methods: HF solvolysis, ELISA, serological methods
Biological activity: serological data, immunoreactivity data
Comments, role: consensus structure; repeating unit HF-released polysaccharide can be sustituted by Gal, Glc as well as by acetyl groups.
Related record ID(s): 23764
NCBI Taxonomy refs (TaxIDs): 1392,
1396
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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