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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: chronic obstructive pulmonary disease [ICD11: CA22

]

NCBI PubMed ID: 19651870
Publication DOI: 10.1128/IAI.00294-09
Journal NLM ID: 0246127
Publisher: American Society for Microbiology
Correspondence: aac

buffalo.edu
Institutions: Department of Microbiology and Immunology, University at Buffalo, Buffalo, NY 14214, USA

Moraxella catarrhalis is a causative agent of otitis media in children and lower respiratory tract infections in adults suffering from chronic obstructive pulmonary disease (COPD). This strict human pathogen continues to be a significant cause of disease in this broad spectrum of patients because there is no available vaccine. Although numerous putative vaccine antigens have been described, little is known about the human immune response to M. catarrhalis infection in vivo. Human serum antibodies are directed at a number of surface proteins, and lipooligosaccharides (LOS) and detoxified LOS may be an effective immunogen in mice. In this study, we used a specific LOS-based enzyme-linked immunosorbent assay (ELISA), containing the three major M. catarrhalis serotypes together with a complete series of truncated LOS mutants, to detect the development of new antibodies to specific regions of the oligosaccharide molecule. We compared serum samples from COPD patients who had recently cleared an M. catarrhalis infection to serum samples collected prior to their infection. Variability in the antibody response to LOS was observed, as some patients developed serotype-specific antibodies, others developed antibodies to the LOS of each serotype, others developed broadly cross-reactive antibodies, and some did not develop new antibodies. These newly developed human antibodies are directed at both side chains and core structures in the LOS molecule. This LOS-based ELISA can be used to dissect the human antibody response to both internal and external carbohydrate epitopes, thus providing a better understanding of the humoral immune response to M. catarrhalis LOS epitopes developed during natural infection.

lipopolysaccharides, Bacterial, antibodies, epitopes, mutation, serum, Moraxella (Branhamella) catarrhalis

Structure type: oligomer
Location inside paper: p.4554, table 4
Aglycon: Kdop-LipidA
Compound class: LOS
Contained glycoepitopes: IEDB_130650,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_141806,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_153543,IEDB_190606,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_178,SB_187,SB_192,SB_195,SB_31,SB_6,SB_62,SB_7,SB_88

Methods: methylation, PCR, de-O-acylation, SDS-PAGE, sugar analysis, ELISA, ESI-MS, GLC, mild acid hydrolysis, serological methods, genetic methods
Biological activity: serological data
Biosynthesis and genetic data: genetic data
Comments, role: mutant LOS

Related record ID(s): 23837, 24217, 24218, 24219, 24220, 24221, 24222, 24223, 24225, 24226, 24227, 24228, 24229
NCBI Taxonomy refs (TaxIDs): 480
Reference(s) to other database(s): GTC:G59797VC, GlycomeDB:37420
Show glycosyltransferases

There is only one chemically distinct structure:

O=C(O)[C@@]1(O)C[C@@H](O)[C@@H](O[C@H]2O[C@H](CO[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O[C@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O[C@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@@H]([C@H](O)CO)O1

SVG MW SMILES 3D mol Ionize