Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: septicemia [ICD11:
MA15.Y 
];
meningitis [ICD11:
1D01 ];
infection due to Neisseria meningitidis [ICD11:
XN1DV ]
The structure was elucidated in this paperNCBI PubMed ID: 19095648Publication DOI: 10.1074/jbc.M808209200Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: johm.cipollo
fda.hhs.gov
Institutions: Center for Biologics Evaluation and Research, United States Food and Drug Administration, Bethesda, MD, USA
Neisseria meningitidis is a cause of fatal sepsis and epidemic meningitis. A major virulence factor is cell wall lipooligosaccharide (LOS). The M986 strain has been used extensively in immunological and vaccine research. Yet, the LOS repertoire of this strain is not known. Here we have investigated the LOS structures of M986 and three of its variants OP1, OP2-, and OP2+. This strain and its variants present a series of related LOS families that are increasingly truncated in their listed order. The major structural differences are seen in the lacto-N-neotetraose alpha-chain. The gamma-chain Hep II contains two phosphoethanolamine (PEA) substitutions at C3 and C6/7. These substitutions were seen in all strains except OP2+ where the canonical core Hep II is missing. The PEA disubstitution was present in nearly stoichiometric amounts with only minor amounts of monosubstitution observed, and no glycomers devoid of PEA were seen. This was also the case in LOS with a complete lacto-N-neotetraosyl alpha-chain even though previous reports suggested that the presence of an extended alpha-chain hinders C3 PEA substitution of Hep II. Approximately 50% of gamma-chain GlcNAc was present in its 3-OAc-substituted form. Because Hep II C3 PEA substitution and gamma-chain GlcNAc OAc addition have been reported to negatively interact, the co-existence of these two modifications in these strains is unique. The LOS structures of M986 and three of its variants have been determined, which better defines these strains as tools for immunological and vaccine research.
Lipooligosaccharide, Neisseria meningitidis, virulence factor
Structure type: oligomer
Location inside paper: p.4624, scheme 2, OP2+
Aglycon: lipid A
Trivial name: outer core region
Compound class: core oligosaccharide, LOS, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_2189047
Methods: 1H NMR, GC-MS, de-O-acylation, sugar analysis, Western blotting, MALDI-TOF MS, NMR-1D, HPLC, LC-MS
Comments, role: OP2+ variant of Neisseria meningitidis strain M986
Related record ID(s): 1785, 7159, 23400, 23863, 24246, 24247, 24941, 122852
NCBI Taxonomy refs (TaxIDs): 487Reference(s) to other database(s): GTC:G38337UV, GlycomeDB:
16228
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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