Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 19040662Publication DOI: 10.1111/j.1574-695X.2008.00494.xJournal NLM ID: 9315554Publisher: Elsevier
Correspondence: wanglei
nankai.edu.cn
Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
The O-antigen, consisting of many repeats of an oligosaccharide, is an essential component of the lipopolysaccharide on the surface of Gram-negative bacteria. The O-antigen is one of the most variable cell constituents, and different O-antigen forms are almost entirely due to genetic variations in O-antigen gene clusters. In this paper, we present structural and genetic evidence for a close relationship between Escherichia coli O107 and E. coli O117 O antigens. The O-antigen of E. coli O107 has a pentasaccharide repeating unit with the following structure: →4)-β-D-GalpNAc-(1→3)-α-L-Rhap-(1→4)-α-D-GlcpNAc-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→, which differs from the known repeating unit of E. coli O117 only in the substitution of D-GlcNAc for D-Glc. The O-antigen gene clusters of E. coli O107 and O117 share 98.6% overall DNA identity and contain the same set of genes in the same organization. It is proposed that one cluster was evolved from another via mutations, and the substitution of a few amino acids residues in predicted glycosyltransferases resulted in the functional change of one such protein for transferring different sugars in O107 (D-GlcNAc) and O117 (D-Glc), leading to different O-antigen structures. This is an example of the O-antigen alteration caused by nucleotide mutations, which is less commonly reported for O-antigen variations.
Lipopolysaccharide, O-antigen, Escherichia coli O107, Escherichia coli O117
Structure type: suggested polymer biological repeating unit
Location inside paper: abstract, p.51, fig. 3
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_141584,IEDB_141794,IEDB_142488,IEDB_143260,IEDB_144998,IEDB_146664,IEDB_190606,IEDB_225177,IEDB_885822,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_23,SB_24,SB_7,SB_8,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, DNA techniques, acid hydrolysis, GLC, genetic methods
Biosynthesis and genetic data: genetic data
Comments, role: biological repeat frame was based on [PMID:19040662]
Related record ID(s): 3145, 20704, 21517, 23872, 28683
NCBI Taxonomy refs (TaxIDs): 2067430Reference(s) to other database(s): GTC:G65895LI, GlycomeDB:
28135
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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