Teichoic acid and disaccharide-1-phosphate polymer were identified in the cell walls of Bacillus subtilis subsp. subtilis VKM B-501(T). The teichoic acid represents 1,3-poly(glycerol phosphate) 80% substituted by α-D-glucopyranose residues at O-2 of glycerol. The linear repeating unit of disaccharide-1-phosphate polymer contains the residues of β-D-glucopyranose, N-acetyl-α-D-galactosamine, and phosphate and has the following structure: -6)-β-D-Glcp-(1→3)-α-D-GalpNAc-(1-P-. The structures of two anionic polymers were determined by chemical and NMR-spectroscopic methods. The 1H- and 13C-NMR spectral data on disaccharide-1-phosphate polymer are presented for the first time.

NMR spectroscopy, cell wall, teichoic acid, Bacillus subtilis, anionic polysaccharides, disaccharide-1-phosphate polymer

13C NMR data:
Linkage	Residue	C1	C2	C3
0	x?Gro	67.7	70.8	67.7
	P


1H NMR data:
Linkage	Residue	H1	H2	H3
0	x?Gro	3.94-3.99	4.06	3.94-3.99
	P


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3
0	x?Gro	67.7/3.94-3.99	70.8/4.06	67.7/3.94-3.99
	P

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*OCC(O)COP(*)(=O)O

154.058 g/mol (C₃H₇R₂O₅P, R = next and previous repeats, not counted in mol weight)