Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: septicemia [ICD11:
MA15.Y 
]
NCBI PubMed ID: 19695563Publication DOI: 10.1016/j.carres.2009.06.035Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: knirel
ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Departamento de Microbiología y Ecología, Universidad de València, 46100 Burjassot, Valencia, Spain, Departamento Microbiología, Facultad Biología, Universidad de Barcelona, 08071 Barcelona, Spain
High-molecular-mass polysaccharides were released by mild acid degradation of the lipopolysaccharides of two wild-type Vibrio vulnificus strain, a flagellated motile strain CECT 5198 and a non-flagellated non-motile strain S3-I2-36. Studies by sugar analysis and partial acid hydrolysis along with (1)H and (13)C NMR spectroscopies showed that the polysaccharides from both strains have the same trisaccharide repeating unit of the following structure: →4)-β-D-GlcpNAc3NAcylAN-(1→4)-α-L-GalpNAmA-(1→3)-α-D-QuipNAc-(1→ where QuiNAc stands for 2-acetamido-2,6-dideoxyglucose, GalNAmA for 2-acetimidoylamino-2-deoxygalacturonic acid, GlcNAc3NAcylAN for 2-acetamido-3-acylamino-2,3-dideoxyglucuronamide and acyl for 4-d-malyl (approximately 30%) or 2-O-acetyl-4-d-malyl (approximately 70%). The structure of the polysaccharide studied resembles much that of a marine bacterium Pseudoalteromonas rubra ATCC 29570 reinvestigated in this work. The latter differs in (i) the absolute configuration of malic acid (l vs d), (ii) 3-O-acetylation of GalNAmA and (iii) replacement of QuiNAc with its 4-keto biosynthetic precursor.
Lipopolysaccharide, bacterial polysaccharide structure, 2, 3-diamino-2, Vibrio vulnificus, N-Acetimidoyl-L-galactosaminuronic acid, 3-dideoxy-d-glucuronamide, malic acid
Structure type: polymer chemical repeating unit
Location inside paper: p.2008, fig.3
Compound class: O-polysaccharide, O-antigen
Methods: 13C NMR, 1H NMR, NMR-2D, partial acid hydrolysis, sugar analysis, GLC, de-O-acetylation, GPC
Comments, role: extracted from seafood, polysaccharide from LPS.
Related record ID(s): 23842, 24236
NCBI Taxonomy refs (TaxIDs): 1117318
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error
(later renamed to: Pseudoalteromonas rubra DSM 6842)