Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Streptococcus pneumoniae [ICD11:
XN3PW 
]
NCBI PubMed ID: 11159969Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: JWO
GBF.de
Institutions: Vaccines Section, Eijkman-Winkler Institute for Microbiology, Infectious Diseases, and Inflammation, Utrecht University Hospital, 3584 CX Utrecht, Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, 3584 CH Utrecht, The Netherlands
The immunogenicity and protective capacity of Streptococcus pneumoniae 6B capsular polysaccharide (PS)-derived synthetic phosphate-containing disaccharide (Rha-ribitol-P-), trisaccharide (ribitol-P-Gal-Glc-), and tetrasaccharide (Rha-ribitol-P-Gal-Glc-)-protein conjugates in rabbits and mice were studied. In rabbits, all saccharides conjugated to keyhole limpet hemocyanin (KLH) evoked high levels of pneumococcal (Pn) type 6B antibodies that facilitated type-specific phagocytosis. Unlike the disaccharide rabbit antisera, tri- and tetrasaccharide rabbit antisera also reacted with 6A PS in an enzyme-linked immunosorbent assay (ELISA) and promoted phagocytosis of 6A pneumococci. All these rabbit antisera passively protected mice against a Pn 6B challenge. The disaccharide conjugate-induced antiserum, however, failed to protect mice against a 6A challenge. In mice, phagocytic and protective anti-Pn 6B antibodies were only induced by the tetrasaccharide conjugate and not by PS 6B or PS 6B-protein conjugates. These antibodies did not cross-react with 6A PS in ELISA and were unable to phagocytize 6A pneumococci. In conclusion, the disaccharide and tetrasaccharide conjugates already contain epitopes capable of inducing 6B-specific, fully protective antibodies in rabbits and mice, respectively.
common, antibodies, antibody, epitope, type, mice, epitopes, specific, Synthetic, pneumococcal, conjugate, conjugates, protective
Structure type: oligomer
Location inside paper: Fig. 1
Aglycon: spacer-keyhole limpet hemocyanin
Trivial name: conjugate
Contained glycoepitopes: IEDB_114703,IEDB_136105,IEDB_153532,IEDB_225177,IEDB_591403,IEDB_885823
Methods: ELISA
Biological activity: immunogenicity,protectivity
Synthetic data: chemical
Related record ID(s): 2537, 2538
NCBI Taxonomy refs (TaxIDs): 1313Reference(s) to other database(s): GTC:G78193GH, GlycomeDB:
25666
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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