Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
Studies by sugar analysis and partial acid hydrolysis along with one- and two-dimensional (1)H and (13)C NMR spectroscopy and high-resolution ESI MS showed that the O-polysaccharide (O-antigen) Cronobacter sakazakii ATCC 29004 (serotype O2) possesses a branched hexasaccharide O-unit with a randomly mono-O-acetylated terminal rhamnose residue in the side chain and the following structure: [Formula: see text]. A similar structure has been reported for the O-polysaccharide of C. sakazakii 767, which differs in the presence of an additional lateral α-D-Glcp residue on GlcNAc and the pattern of O-acetylation (Czerwicka, M., Forsythe, S. J.; Bychowska, A.; Dziadziuszko, H.; Kunikowska, D.; Stepnowski, P.; Kaczynski, Z. Carbohydr. Res.2010, 345, 908-913).
13C NMR, 1H NMR, NMR-2D, partial acid hydrolysis, sugar analysis, ESI-MS, NMR-1D
The O-acetyl groups are distributed between positions 2, 3, and 4 almost equally and the total degree of O-acetylation is 70%.
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2,4,2,4,2 %Ac
3,2,4,2,4,3 %Ac
3,2,4,2,4,4 %Ac
3,2,4,2,4 aLRhap 101.8 71.9 71.5 73.7 70.1 17.8
3,2,4,2 aDGalpA 99.0 69.4 72.1 77.3 73.3 175.9
3,2,4 aLRhap 97.4 75.3 80.7 72.4 71.0 18.2
3,2 aDGlcp 99.1 73.1 73.1 78.7 71.9 61.4
3 aLRhap 99.9 78.4 70.8 73.3 70.6 17.8
2 Ac 175.7 23.8
bDGlcpN 104.3 57.5 82.7 69.9 77.3 62.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2,4,2,4,2 %Ac
3,2,4,2,4,3 %Ac
3,2,4,2,4,4 %Ac
3,2,4,2,4 aLRhap 5.30 4.08 3.84 3.37 3.83 1.27
3,2,4,2 aDGalpA 5.03 3.94 4.12 4.52 4.69 -
3,2,4 aLRhap 5.03 4.31 4.11 3.58 4.04 1.28
3,2 aDGlcp 4.87 3.57 3.85 3.66 4.10 3.82-3.82
3 aLRhap 4.97 3.87 3.91 3.48 4.03 1.27
2 Ac - 2.07
bDGlcpN 4.68 3.74 3.68 3.52 3.38 3.77-3.87
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2,4,2,4,2 %Ac
3,2,4,2,4,3 %Ac
3,2,4,2,4,4 %Ac
3,2,4,2,4 aLRhap 101.8/5.30 71.9/4.08 71.5/3.84 73.7/3.37 70.1/3.83 17.8/1.27
3,2,4,2 aDGalpA 99.0/5.03 69.4/3.94 72.1/4.12 77.3/4.52 73.3/4.69
3,2,4 aLRhap 97.4/5.03 75.3/4.31 80.7/4.11 72.4/3.58 71.0/4.04 18.2/1.28
3,2 aDGlcp 99.1/4.87 73.1/3.57 73.1/3.85 78.7/3.66 71.9/4.10 61.4/3.82-3.82
3 aLRhap 99.9/4.97 78.4/3.87 70.8/3.91 73.3/3.48 70.6/4.03 17.8/1.27
2 Ac 23.8/2.07
bDGlcpN 104.3/4.68 57.5/3.74 82.7/3.68 69.9/3.52 77.3/3.38 62.6/3.77-3.87
report error
Found 1 record.
Displayed record 1
(previously named: Enterobacter sakazakii O2 ATCC 29004)
]; neonatal necrotising enterocolitis [ICD11: KB88 
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