Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: bacteremia [ICD11:
MA15.0 
];
neonatal necrotising enterocolitis [ICD11:
KB88 , Life stage: neonatal];
neonatal meningitis [ICD11:
KA65.4 , Life stage: neonatal]
The structure was elucidated in this paperNCBI PubMed ID: 20719304Publication DOI: 10.1016/j.carres.2010.07.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Y.A.Knirel <yknirel
gmail.com>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
The O-polysaccharide (O-antigen) was released by mild acid hydrolysis of the lipopolysaccharide of Cronobacter sakazakii ATCC 29544(T) (serotype O1) and studied by composition analysis and Smith degradation, in addition to 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established: [Formula: see text] where d-Qui3NAcyl stands for 3-(N-acetyl-l-alanyl)amino-3,6-dideoxy-d-glucose. The same composition but a different structure has been reported earlier for the O-polysaccharide of C. sakazakii 3290 [MacLean, L. L.; Pagotto, F.; Farber, J. M.; Perry, M. B. Biochem. Cell Biol.2009, 87, 459-465].
Lipopolysaccharide, O-antigen, 6-dideoxy-d-glucose, bacterial polysaccharide structure, 3-amino-3, Cronobacter sakazakii
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.2098, chart 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_140630,IEDB_141584,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_885822,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, Smith degradation, NMR-1D
Related record ID(s): 25508
NCBI Taxonomy refs (TaxIDs): 28141
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,6,3,2 Ac
3,6,3 xLAla? 177.7 51.0 18.8
3,6 bDQuip3N 104.8 73.6 57.5 74.4 74.6 18.3
3,4,4 aDGlcp 102.7 73.2 74.5 70.9 74.1 62.1
3,4 aDGlcpA 99.4 72.5 74.1 82.0 70.3 175.1
3 bDGlcp 105.9 74.1 77.7 77.6 76.8 69.0
2 Ac 175.1 23.3
aDGalpN 96.4 49.2 78.8 70.1 72.8 62.8
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,6,3,2 Ac
3,6,3 xLAla? - 4.36 1.30
3,6 bDQuip3N 4.72 3.56 3.94 3.28 3.55 1.32
3,4,4 aDGlcp 5.06 3.55 3.70 3.39 3.77 3.78-3.85
3,4 aDGlcpA 5.47 3.51 3.90 3.35 3.87 -
3 bDGlcp 4.44 3.41 3.73 3.78 3.88 3.77-4.10
2 Ac - 2.03
aDGalpN 5.58 4.40 3.63 4.13 3.76 3.69-3.82
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,6,3,2 Ac
3,6,3 xLAla? 51.0/4.36 18.8/1.30
3,6 bDQuip3N 104.8/4.72 73.6/3.56 57.5/3.94 74.4/3.28 74.6/3.55 18.3/1.32
3,4,4 aDGlcp 102.7/5.06 73.2/3.55 74.5/3.70 70.9/3.39 74.1/3.77 62.1/3.78-3.85
3,4 aDGlcpA 99.4/5.47 72.5/3.51 74.1/3.90 82.0/3.35 70.3/3.87
3 bDGlcp 105.9/4.44 74.1/3.41 77.7/3.73 77.6/3.78 76.8/3.88 69.0/3.77-4.10
2 Ac 23.3/2.03
aDGalpN 96.4/5.58 49.2/4.40 78.8/3.63 70.1/4.13 72.8/3.76 62.8/3.69-3.82
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,6,3,2 | Ac | |
| 3,6,3 | xLAla? |
| 4.36 | 1.30 | |
| 3,6 | bDQuip3N | 4.72 | 3.56 | 3.94 | 3.28 | 3.55 | 1.32 |
| 3,4,4 | aDGlcp | 5.06 | 3.55 | 3.70 | 3.39 | 3.77 | 3.78
3.85 |
| 3,4 | aDGlcpA | 5.47 | 3.51 | 3.90 | 3.35 | 3.87 |
|
| 3 | bDGlcp | 4.44 | 3.41 | 3.73 | 3.78 | 3.88 | 3.77
4.10 |
| 2 | Ac |
| 2.03 | |
| | aDGalpN | 5.58 | 4.40 | 3.63 | 4.13 | 3.76 | 3.69
3.82 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,6,3,2 | Ac | |
| 3,6,3 | xLAla? | 177.7 | 51.0 | 18.8 | |
| 3,6 | bDQuip3N | 104.8 | 73.6 | 57.5 | 74.4 | 74.6 | 18.3 |
| 3,4,4 | aDGlcp | 102.7 | 73.2 | 74.5 | 70.9 | 74.1 | 62.1 |
| 3,4 | aDGlcpA | 99.4 | 72.5 | 74.1 | 82.0 | 70.3 | 175.1 |
| 3 | bDGlcp | 105.9 | 74.1 | 77.7 | 77.6 | 76.8 | 69.0 |
| 2 | Ac | 175.1 | 23.3 | |
| | aDGalpN | 96.4 | 49.2 | 78.8 | 70.1 | 72.8 | 62.8 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Enterobacter sakazakii O1 ATCC 29544)