Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter [ICD11:
XN25B 
]
The structure was elucidated in this paperNCBI PubMed ID: 20732679Publication DOI: 10.1016/j.carres.2010.07.031Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: annakond
gmail.com
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
An O-polysaccharide was released by mild acid degradation of the lipopolysaccharide of Acinetobacter sp. VS-15 and studied by chemical methods along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC and HMBC experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established: [Formula: see text]. The O-polysaccharide of Acinetobacter lwoffii EK67 was found to have the same structure.
Lipopolysaccharide, O-antigen, Acinetobacter, bacterial polysaccharide structure, Acinetobacter lwoffii
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.2289
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136105,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391965,IEDB_141584,IEDB_141794,IEDB_141807,IEDB_144989,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_225177,IEDB_423113,IEDB_885822,IEDB_885823,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, Smith degradation, NMR-1D
NCBI Taxonomy refs (TaxIDs): 472,
28090Reference(s) to other database(s): GTC:G97225ZC, GlycomeDB:
37792
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,6 aLRhap 98.7 77.8 70.9 73.5 70.3 18.2
3,4 aDGalp 100.0 70.1 70.5 70.3 69.2 65.5
3,2 Ac 175.1 23.7
3,3,2 Ac 175.0 23.7
3,3 bDGlcpN 104.9 56.6 75.3 71.8 77.0 62.1
3 aDGalpN 98.6 50.3 76.9 75.3 73.1 61.5
2 Ac 175.6 23.7
aDQuipN
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,6 aLRhap 4.78 4.03 3.89 3.48 3.82 1.32
3,4 aDGalp 4.99 3.82 3.98 4.10 4.71 3.61-3.89
3,2 Ac - 2.07
3,3,2 Ac - 1.96
3,3 bDGlcpN 4.47 3.77 3.53 3.31 3.40 3.76-3.89
3 aDGalpN 5.43 4.34 3.80 4.29 3.95 3.80-3.84
2 Ac - 2.07
aDQuipN
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,6 aLRhap 98.7/4.78 77.8/4.03 70.9/3.89 73.5/3.48 70.3/3.82 18.2/1.32
3,4 aDGalp 100.0/4.99 70.1/3.82 70.5/3.98 70.3/4.10 69.2/4.71 65.5/3.61-3.89
3,2 Ac 23.7/2.07
3,3,2 Ac 23.7/1.96
3,3 bDGlcpN 104.9/4.47 56.6/3.77 75.3/3.53 71.8/3.31 77.0/3.40 62.1/3.76-3.89
3 aDGalpN 98.6/5.43 50.3/4.34 76.9/3.80 75.3/4.29 73.1/3.95 61.5/3.80-3.84
2 Ac 23.7/2.07
aDQuipN
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,6 | aLRhap | 4.78 | 4.03 | 3.89 | 3.48 | 3.82 | 1.32 |
| 3,4 | aDGalp | 4.99 | 3.82 | 3.98 | 4.10 | 4.71 | 3.61
3.89 |
| 3,2 | Ac |
| 2.07 | |
| 3,3,2 | Ac |
| 1.96 | |
| 3,3 | bDGlcpN | 4.47 | 3.77 | 3.53 | 3.31 | 3.40 | 3.76
3.89 |
| 3 | aDGalpN | 5.43 | 4.34 | 3.80 | 4.29 | 3.95 | 3.80
3.84 |
| 2 | Ac |
| 2.07 | |
| | aDQuipN | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,6 | aLRhap | 98.7 | 77.8 | 70.9 | 73.5 | 70.3 | 18.2 |
| 3,4 | aDGalp | 100.0 | 70.1 | 70.5 | 70.3 | 69.2 | 65.5 |
| 3,2 | Ac | 175.1 | 23.7 | |
| 3,3,2 | Ac | 175.0 | 23.7 | |
| 3,3 | bDGlcpN | 104.9 | 56.6 | 75.3 | 71.8 | 77.0 | 62.1 |
| 3 | aDGalpN | 98.6 | 50.3 | 76.9 | 75.3 | 73.1 | 61.5 |
| 2 | Ac | 175.6 | 23.7 | |
| | aDQuipN | |
|
There is only one chemically distinct structure:
Found 
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