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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]; infection due to Klebsiella pneumoniae [ICD11: XN741

]

NCBI PubMed ID: 15184370
Publication DOI: 10.1074/jbc.M404738200
Journal NLM ID: 2985121R
Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: cwhitfie

uoguelph.ca
Institutions: Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada

The chain length of bacterial lipopolysaccharide O antigens is regulated to give a modal distribution that is critical for pathogenesis. This paper describes the process of chain length determination in the ATP-binding cassette (ABC) transporter-dependent pathway, a pathway that is widespread among Gram-negative bacteria. Escherichia coli O8 and O9/O9a polymannans are synthesized in the cytoplasm, and an ABC transporter exports the nascent polymer across the inner membrane prior to completion of the LPS molecule. The polymannan O antigens have nonreducing terminal methyl groups. The 3-O-methyl group in serotype O8 is transferred from S-adenosylmethionine by the WbdD(O8) enzyme, and this modification terminates polymerization. Methyl groups are added to the O9a polymannan in a reaction dependent on preceding phosphorylation. The bifunctional WbdD(O9a) catalyzes both reactions, but only the kinase activity controls chain length. Chain termination occurs in a mutant lacking the ABC transporter, indicating that it precedes export. An E. coli wbdD(O9a) mutant accumulated O9a polymannan in the cytoplasm, indicating that WbdD activity coordinates polymannan chain termination with export across the inner membrane.

serotype, Escherichia coli, O-antigens, structure-activity relationship, ABC transporter, chain length

Structure type: oligomer
Location inside paper: p.35711, fig.1A
Aglycon: core
Trivial name: polymannan
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_137340,IEDB_140116,IEDB_141111,IEDB_141795,IEDB_141807,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_145662,IEDB_151531,IEDB_152206,IEDB_153756,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_164480,IEDB_174840,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72

Methods: serological methods, genetic methods, biochemical methods, immunofluorescence microscopy
Biosynthesis and genetic data: genetic data, biochemical data

Related record ID(s): 883, 5762, 7199, 10527, 20624, 25285, 25286, 25287
NCBI Taxonomy refs (TaxIDs): 1010797, 573
Reference(s) to other database(s): GTC:G12878ZR, GlycomeDB:25318
Show glycosyltransferases

There are 4 chemically distinct structures. Please, select:

CO[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O[C@@H]2[C@H](O)[C@@H](O[C@H]3[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]3O[C@H]3[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]3O[C@H]3[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]3O[C@@H]3[C@H](O)[C@@H](O[C@@H]4[C@H](O)[C@@H](O[C@H]5[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]5O[C@H]5[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]5O[C@H]5[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]5O[C@@H]5[C@H](O)[C@@H](O[C@@H]6[C@H](O)[C@@H](O[C@H]7[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]7O[C@@H]7[C@H](O)[C@@H](O[C@H]8[C@H](O)[C@@H](CO)O[C@H](O)[C@@H]8NC(C)=O)O[C@H](CO)[C@H]7O)O[C@H](CO)[C@H]6O)O[C@H](CO)[C@H]5O)O[C@H](CO)[C@H]4O)O[C@H](CO)[C@H]3O)O[C@H](CO)[C@H]2O)O[C@H](CO)[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDManp: unknown unit count was assumed to be 3