Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: functional gastrointestinal disorders [ICD11:
DD9Y 
]
NCBI PubMed ID: 20816206Publication DOI: 10.1016/S0076-6879(10)80005-9Journal NLM ID: 0212271Correspondence: mikael.skurnik
helsinki.fi
Institutions: Universita di Napoli Federico II, Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte Santangelo, Via Cynthia, Napoli, Italy
Bacterial lipopolysaccharides (LPSs) are the major component of the outer membrane of Gram-negative bacteria. They have a structural role since they contribute to the cellular rigidity by increasing the strength of cell wall and mediating contacts with the external environment that can induce structural changes to allow life in different conditions. Furthermore, the low permeability of the outer membrane acts as a barrier to protect bacteria from host-derived antimicrobial compounds. They also have a very important role in the elicitation of the animal and plant host innate immunity since they are microbe-associated molecular patterns, namely, they are glycoconjugates produced only by Gram-negative bacteria and are recognized as a molecular hallmark of invading microbes. LPSs are amphiphilic macromolecules generally comprising three defined regions distinguished by their genetics, structures, and function: the lipid A, the core oligosaccharide and a polysaccharide portion, the O-chain. In some Gram-negative bacteria, LPS can terminate with the core portion to form rough-type LPS (R-LPS, LOS). In this chapter, we will describe the isolation of both kinds of LPSs and their full chemical analysis, pivotal operations in the complete description of the primary structure of such important glycoconjugates.
lipopolysaccharides, isolation, gram negative bacteria, methods, MS, chemical analysis
Structure type: polymer chemical repeating unit
Location inside paper: p.355, fig.2
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_136045,IEDB_137340,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_145669,IEDB_151531,IEDB_152206,IEDB_152214,IEDB_174333,IEDB_983930,SB_44,SB_67,SB_72,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, NMR-1D, genetic methods
Biosynthesis and genetic data: genetic data
Related record ID(s): 25592, 25593
NCBI Taxonomy refs (TaxIDs): 109458Reference(s) to other database(s): GTC:G56990JL, GlycomeDB:
37797
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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