Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 12923092Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: komano-teruya
c.metro-u.ac.jp
Institutions: Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan
IncI1 plasmid R64 encodes a type IV pilus called a thin pilus, which includes PilV adhesins. Seven different sequences for the C-terminal segments of PilV adhesins can be produced by shufflon DNA rearrangement. The expression of the seven PilV adhesins determines the recipient specificity in liquid matings of plasmid R64. Salmonella enterica serovar Typhimurium LT2 was recognized by the PilVA' and PilVB' adhesins, while Escherichia coli K-12 was recognized by the PilVA', PilVC, and PilVC' adhesins. Lipopolysaccharide (LPS) on the surfaces of recipient cells was previously shown to be the specific receptor for the seven PilV adhesins. To identify the specific receptor structures of LPS for various PilV adhesins, R64 liquid matings were carried out with recipient cells consisting of various S. enterica serovar Typhimurium LT2 and E. coli K-12 waa mutants and their derivatives carrying various waa genes of different origins. From the mating experiments, including inhibition experiments, we propose that the GlcNAc(α1-2)Glc and Glc(α1-2)Gal structures of the LPS core of S. enterica serovar Typhimurium LT2 function as receptors for the PilVB' and PilVC' adhesins, respectively, while the PilVC' receptor in the wild-type LT2 LPS core may be masked. We further propose that the GlcNAc(β1-7)Hep and Glc(α1-2)Glc structures of the LPS core of E. coli K-12 function as receptors for the PilVC and PilVC' adhesins, respectively.
Lipopolysaccharide, genetic, lipopolysaccharides, LPS, structure, core, Bacterial Proteins, expression, gene, Bacterial, genetics, metabolism, DNA, Support, molecule, Escherichia, Escherichia coli, type, wild type, specific, mutant, mutants, specificity, Salmonella, plasmid, Salmonella enterica, surface, Salmonella typhimurium, sequence, function, inhibition, virology, recombination, rearrangement, adhesin, LPS core, Typhimurium, receptors, Bacteriophages, Conjugation, Fimbriae, origin, physiology, Plasmids, receptor
Structure type: oligomer
Location inside paper: Fig. 1C
Aglycon: core-Lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_2189047,IEDB_232584,IEDB_983931,SB_192
Methods: SDS-PAGE
Biological activity: adhesin receptor
Biosynthesis and genetic data: genetic data
Related record ID(s): 2465
NCBI Taxonomy refs (TaxIDs): 83333Reference(s) to other database(s): GTC:G19100BZ, GlycomeDB:
25686
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error