The structure was elucidated in this paper NCBI PubMed ID:20227685 Publication DOI:10.1016/j.carres.2010.01.022 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: macua.pt Institutions: Departamento de Química, Universidade de Aveiro, Aveiro, Portugal, LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal, Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga, Portugal, Institute of Molecular Pathology and Immunology, University of Porto, Portugal, Medical Faculty of Porto, Porto, Portugal, CICECO, University of Aveiro, Aveiro, Portugal, Department of Chemistry, University of Guelph, Guelph, ON, Canada
With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both α- and β-configurations: [abstract: see text]. In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host-microbial interactions and immunological modulation.
The structure was elucidated in this paper NCBI PubMed ID:20227685 Publication DOI:10.1016/j.carres.2010.01.022 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: macua.pt Institutions: Departamento de Química, Universidade de Aveiro, Aveiro, Portugal, LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal, Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga, Portugal, Institute of Molecular Pathology and Immunology, University of Porto, Portugal, Medical Faculty of Porto, Porto, Portugal, CICECO, University of Aveiro, Aveiro, Portugal, Department of Chemistry, University of Guelph, Guelph, ON, Canada
With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both α- and β-configurations: [abstract: see text]. In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host-microbial interactions and immunological modulation.
Methods: 13C NMR, 1H NMR, NMR-2D, 31P NMR, composition analysis, affinity chromatography Comments, role: O-chains contains 3.4% of terminal Fuc and 4.0% of O-3,4-linked GlcNAc
Related record ID(s): 288, 3527, 3588, 6391, 21870, 25245, 25599 NCBI Taxonomy refs (TaxIDs):210 Reference(s) to other database(s): GTC:G39453SO, GlycomeDB:15913 Show glycosyltransferases
The structure was elucidated in this paper NCBI PubMed ID:20227685 Publication DOI:10.1016/j.carres.2010.01.022 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: macua.pt Institutions: Departamento de Química, Universidade de Aveiro, Aveiro, Portugal, LEPAE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal, Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga, Portugal, Institute of Molecular Pathology and Immunology, University of Porto, Portugal, Medical Faculty of Porto, Porto, Portugal, CICECO, University of Aveiro, Aveiro, Portugal, Department of Chemistry, University of Guelph, Guelph, ON, Canada
With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both α- and β-configurations: [abstract: see text]. In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host-microbial interactions and immunological modulation.
report error
Found 3 records.
Displayed records from 1 to 3
]; infection due to Helicobacter pylori [ICD11: XN3DY 
ua.pt