Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
The structure was elucidated in this paperNCBI PubMed ID: 19880661Journal NLM ID: 101469670Publisher: Sage Publications
Correspondence: molinaro
unina.it
Institutions: Dipartimento di Chimica Organica e Biochimica, Università degli Studi di Napoli Federico, Napoli, Italy, Dipartimento di Scienze della Vita, Università degli Studi di Trieste, Trieste, Italy, Institute of Pediatrics, University of Foggia and Dynamic Imaging Microscopy, CEINGE, Naples, Italy, Department of Pediatrics, Università degli Studi di Napoli Federico II, Naples, Italy, Institute of Pediatrics, University of Foggia and European Institute for Research in Cystic Fibrosis, San Raffaele Scientific Institute, Milan, Italy
Cystic fibrosis is an autosomal recessive disorder and it is characterised by chronic bacterial airway infection which leads to progressive lung deterioration, sometimes with fatal outcome. Burkholderia multivorans and Burkholderia cenocepacia are the species responsible for most of the infections of cystic fibrosis patients. Lipopolysaccharide endotoxins (LPSs) are among the foremost factors of pathogenesis of Gram-negative infection and, in particular, lipid A is the endotoxic portion of LPS responsible for eliciting host innate immune response. In this work, the complete primary structure of the lipid A from B. multivorans C1576 has been defined and, further, its pro-inflammatory activity in a cystic fibrosis airways model is shown. The structure of B. multivorans lipid A was attained by chemical, mass spectrometry and nuclear magnetic resonance analyses whereas its biological activity was assessed on the intestinal epithelial cell line CACO-2 cells, on the airway epithelial IB3-1 cells, carrying the ∆F508/W1282X CFTR mutation and on an ex vivo model of culture explants of nasal polyps.
Lipopolysaccharide, lipid A, mass spectrometry, cystic fibrosis, Burkholderia multivorans
Structure type: oligomer
Location inside paper: p.361, fig.4
Compound class: lipid A
Contained glycoepitopes: IEDB_141807,IEDB_151531,IEDB_176772
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, GC-MS, de-O-acylation, SDS-PAGE, sugar analysis, TLC, ELISA, ESI-MS, mild acid hydrolysis, biological assays
NCBI Taxonomy refs (TaxIDs): 87883
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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