Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Homo sapiens
Associated disease: skin infections [ICD11:
1B7Y 
, ICD11:
1C41 , ICD11:
1C44 ];
septicemia [ICD11:
MA15.Y ];
necrotising fasciitis [ICD11:
1B71 ];
toxic shock syndrome [ICD11:
1B52 ];
infection due to Streptococcus [ICD11:
XN3NM ]
NCBI PubMed ID: 20870056Publication DOI: 10.1016/j.vaccine.2010.09.018Journal NLM ID: 8406899Publisher: Elsevier
Correspondence: P. Costantino <paolo.costantino
novartis.com>
Institutions: Research Center, Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
Bacterial infections caused by Group A Streptococcus (GAS) are a serious health care concern that currently cannot be prevented by vaccination. The GAS cell-wall polysaccharide (GAS-PS) is an attractive vaccine candidate due to its constant expression pattern on different bacterial strains and protective properties of anti-GAS-PS antibodies. Here we report for the first time the immunoprotective efficacy of glycoconjugates with synthetic GAS oligosaccharides as compared to those containing the native GAS-PS. A series of hexa- and dodecasaccharides based on the GAS-PS structure were prepared by chemical synthesis and conjugated to CRM(197). When tested in mice, the conjugates containing the synthetic oligosaccharides conferred levels of immunoprotection comparable to those elicited by the native conjugate. Antisera from immunized rabbits promoted phagocytosis of encapsulated GAS strains. Furthermore we discuss variables that might correlate with glycoconjugate immunogenicity and demonstrate the potential of the synthetic approach that benefits from increased antigen purity and facilitated manufacturing.
carbohydrate, GAS, Group A Streptococcus, CHO, wt, weight, ip, intraperitoneal, EU, ELISA units
Structure type: polymer chemical repeating unit
Location inside paper: p.105, fig.1a
Trivial name: GAS-PS, GAC, GAC, group A carbohydrate, GAC-PS
Compound class: cell wall polysaccharide
Contained glycoepitopes: IEDB_127513,IEDB_131174,IEDB_131175,IEDB_131177,IEDB_133754,IEDB_135610,IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_143254,IEDB_151531,IEDB_2218296,IEDB_225177,IEDB_885823
Methods: 1H NMR, ELISA, serological methods, immunization, conjugation, MALDI-TOF/TOF MS
Biological activity: immunoprotective properties of glycoconjugates
Synthetic data: chemical
Related record ID(s): 25709, 25710, 25711, 25712
NCBI Taxonomy refs (TaxIDs): 1301Reference(s) to other database(s): GTC:G59445KA, GlycomeDB:
37806
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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