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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: enterocolitis [ICD11: 1A40.Z

]; septicemia [ICD11: MA15.Y

]; meningitis [ICD11: 1D01

]

NCBI PubMed ID: 20684949
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: M.B. Perry <malcolm.perry

nrc-cnrc.gc.ca>
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Canada

Strains of Cronobacter sakazakii (previously known as Enterobactersakazakii) are medically recognized important Gram-negative bacterial pathogens that cause enterocolitis, septicemia, and meningitis, with a high mortality rate in neonates. The structure of their O-antigens, that form part of their somatic lipopolysaccharide (LPS) components, is of interest for their chemical and serological identification and their relationship to virulence. The O-polysaccharide (O-PS) of C.sakazakii HPB 2855 (SK 81), a strain isolated from an infant at the Hospital for Sick Children in Toronto in 1981, was shown to be a polymer of a partially O-acetylated-repeating hexasaccharide unit composed of d-glucose, d-galacturonic acid, 2-acetamido-2-deoxy-d-galactose, and l-rhamnose (1:1:1:3). From composition and methylation analysis, and the application of 1D and 2D (1)H and (13)C NMR spectroscopy, the O-PS was determined to be a polymer of a repeating oligosaccharide unit having the structure: (formula, see text).

Lipopolysaccharide, polysaccharide, O-antigen, NMR spectroscopy, Cronobacter sakazakii

Structure type: polymer chemical repeating unit
Location inside paper: p.1937, O-PS [II]
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

Methods: 13C NMR, 1H NMR, methylation, GLC-MS, sugar analysis, GLC, mild acid hydrolysis, DOC-PAGE, de-O-acetylation, NMR-1D, CE-MS

Related record ID(s): 25223, 25756, 25757
NCBI Taxonomy refs (TaxIDs): 28141
Reference(s) to other database(s): GTC:G20299ZC
Show glycosyltransferases

There is only one chemically distinct structure:

*O[C@H]1[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](OC(C)=O)[C@H](C)O[C@@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@H]4[C@H](*)O[C@@H](C)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@@H]2O[C@H]2O[C@H](C(=O)O)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@@H]1NC(C)=O

SVG MW SMILES 3D mol Ionize