Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: tularemia [ICD11:
1B94 
, ICD11:
XN0BX ];
infection due to Francisella tularensis [ICD11:
XN0BX ]
NCBI PubMed ID: 20618133Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Correspondence: mokrievich
obolensk.org
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia
A knockout mutant with a deletion in a quorum sensing system gene qseC was generated from the vaccine strain Francisella tularensis 15 by site-directed mutagenesis. The variant with the inactivated gene qseC differed from the parental strain in growth rate on solid nutrient medium but had the same growth dynamics in liquid nutrient medium. The mutation abolished almost completely the resistance of the vaccine strain to normal rabbit serum and its ability to survive in macrophages; in addition, the strain lost the residual virulence. A significant phenotypic alteration was observed in the lipopolysaccharide of F. tularensis. Particularly, the mutant strain synthesized no noticeable amount of the lipopolysaccharide with the high-molecular-mass O-polysaccharide, presumably as a result of impairing biosynthesis of the repeating unit, namely, a loss of the ability to incorporate a formyl group, an N-acyl substituent of 4-amino-4,6-dideoxy-D-glucose.
Lipopolysaccharide, Francisella tularensis, tularemia, quorum sensing, gene qseC, sitedirected mutagenesis
Structure type: polymer chemical repeating unit
Location inside paper: p.448, fig.3, (1)
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_151083,IEDB_221847,IEDB_221848
Methods: SDS-PAGE, DNA techniques, mild acid hydrolysis, ESI-ICR-MS, Western blotting, genetic methods
Biological activity: phagocytosis, virulence, biological properties
Biosynthesis and genetic data: genetic data
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 25780, 25781, 25782
NCBI Taxonomy refs (TaxIDs): 263Reference(s) to other database(s): GTC:G66306WB, GlycomeDB:
3488
Show glycosyltransferases
There is only one chemically distinct structure:
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