Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11:
XN3LD 
];
infection due to Pseudomonas pseudomallei [ICD11:
XN8AA ]
NCBI PubMed ID: 20724509Publication DOI: 10.1099/jmm.0.022202-0Journal NLM ID: 0224131Publisher: Reading, England: Society for General Microbiology
Correspondence: shaunaz
athabascau.ca
Institutions: Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Calgary, AB T2N 4N1, Canada
Burkholderia pseudomallei has been shown to produce more than one capsular polysaccharide (CPS). Analysis of the B. pseudomallei genome has revealed that the organism contains four CPS operons (I-IV). One of these operons (CPS III) was selected for further study. Comparative sequencing analysis revealed that the genes encoding CPS III are present in B. pseudomallei and Burkholderia thailandensis but not in Burkholderia mallei. In this study, CPS III was not found to contribute to the virulence of B. pseudomallei. Strains containing mutations in CPS III had the same LD(50) value as the wild-type when tested in an animal infection model. Production of CPS III was shown to be induced in water but inhibited in 30% normal human serum using a lux reporter fusion assay. Microarray analysis of capsule gene expression in infected hamsters revealed that the genes encoding CPS III were not significantly expressed in vivo compared with the genes encoding the previously characterized mannoheptose capsule (CPS I), which is an important virulence factor in B. pseudomallei. Glycosyl-composition analysis by combined GC/MS indicated that the CPS III genes are involved in the synthesis of a capsule composed of galactose, glucose, mannose and xylose.
capsular polysaccharide, Burkholderia pseudomallei, gene expression, virulence factor, Bacterial Capsules, Burkholderia mallei
Structure type: polymer chemical repeating unit
Location inside paper: p.1412
Trivial name: K-antigen, galactan-Kdo, Kdo-EPS
Compound class: CPS, EPS
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_36,SB_7,SB_87,SB_88,SB_9
Methods: GC-MS, DNA techniques, Western blotting, composition analysis, genetic methods
Biosynthesis and genetic data: genetic data
Related record ID(s): 864, 3412, 3773
NCBI Taxonomy refs (TaxIDs): 28450Reference(s) to other database(s): GTC:G77110LE, GlycomeDB:
25312
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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(previously named: Pseudomonas pseudomallei)