Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: acute otitis media [ICD11:
AB00 
, Life stage: adult];
infection due to Haemophilus influenzae [ICD11:
XN1P6 ]
NCBI PubMed ID: 19882122Publication DOI: 10.1007/978-1-60761-454-8_6Publisher: Totowa, NJ: Humana Press
Editors: Holst O, Walker JM, Beck A
Institutions: Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada, Clinical Research Centre, Karolinska Institute and Södertörn University, Huddinge, Sweden
Non-typeable (acapsular) Haemophilus influenzae (NTHi) is a major cause of otitis media accounting for 25-30% of all cases of the disease. Lipopolysaccharide (LPS) is an essential and exposed component of the H. influenzae cell wall. A characteristic feature of H. influenzae LPS is the extensive inter-strain and intra-strain heterogeneity of glycoform structure which is key to the role of the molecule in both commensal and disease-causing behavior of the bacterium. However, to characterize LPS structure unambiguously is a major challenge due to the extreme heterogeneity of glycoforms that certain strains express. A powerful tool for obtaining sequence and branching information is multiple-stage tandem ESI-MS (ESI-MS( n )) performed on dephosphorylated and permethylated oligosaccharide material using an ESI-quadrupole ion trap mass spectrometer. In general, permethylation increases the MS response by several orders of magnitude and sequence information is readily obtained since methyl tagging allows the distinction between fragment ions generated by cleavage of a single glycosidic bond and inner fragments resulting from the rupture of two glycosidic linkages. Using this approach we are now able to identify all isomeric glycoforms in very heterogeneous LPS preparations.
Lipopolysaccharide, Haemophilus influenzae, multiple-stage tandem mass spectrometry (MS(n)), isomeric glycoforms
Structure type: oligomer
Location inside paper: p.80, fig.6.1
Aglycon: (2->6)lipid A
Trivial name: conserved tri-heptosyl inner core
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_137777,IEDB_138949,IEDB_140088,IEDB_2189047
Methods: ESI-MS, HPLC
NCBI Taxonomy refs (TaxIDs): 727
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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