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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: urinary tract infections (UTI) [ICD11: GC08

]

NCBI PubMed ID: 20622068
Publication DOI: 10.1128/JB.00494-10
Journal NLM ID: 2985120R
Publisher: American Society for Microbiology
Correspondence: jtomas

ub.edu
Institutions: Departamento de Microbiologia, Facultad de Biologia, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain, Departamento de Microbiologia y Parasitologia Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain

In this study, we report the identification of genes required for the biosynthesis of the core lipopolysaccharides (LPSs) of two strains of Proteus mirabilis. Since P. mirabilis and Klebsiella pneumoniae share a core LPS carbohydrate backbone extending up to the second outer-core residue, the functions of the common P. mirabilis genes was elucidated by genetic complementation studies using well-defined mutants of K. pneumoniae. The functions of strain-specific outer-core genes were identified by using as surrogate acceptors LPSs from two well-defined K. pneumoniae core LPS mutants. This approach allowed the identification of two new heptosyltransferases (WamA and WamC), a galactosyltransferase (WamB), and an N-acetylglucosaminyltransferase (WamD). In both strains, most of these genes were found in the so-called waa gene cluster, although one common core biosynthetic gene (wabO) was found outside this cluster.

biosynthesis, lipopolysaccharide biosynthesis, gene cluster, Proteus mirabilis, transferases

Structure type: oligomer
Location inside paper: p.4414, fig.1
Aglycon: (2->6)lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130670,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141806,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_2189046,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192

Methods: MALDI-TOF MS, genetic methods, SDS-Tricine-PAGE
Biosynthesis and genetic data: genetic data
Comments, role: HEP = a-Hep

Related record ID(s): 5687, 25177, 25501, 25502, 25503
NCBI Taxonomy refs (TaxIDs): 615
Reference(s) to other database(s): GlycomeDB:26758
Show glycosyltransferases

There is only one chemically distinct structure:

[1*]O[C@H]1[C@@H](O)[C@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H]1[C@@H](O[C@@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](O)[C@@H](O[C@@H]4[C@H](O[C@]5(C(=O)O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O5)C[C@](O)(C(=O)O)O[C@@H]4[C@H](O)CO)O[C@H]([C@@H](O)CO)[C@H]3O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)O[C@H]([C@@H](O)CO[C@H]3O[C@H]([C@@H](O)CO)[C@@H](O)[C@H](O)[C@@H]3O)[C@H]2O)O[C@H](C(=O)O)[C@H](OC2O[C@H](CO)[C@@H](O[C@H]3O[C@H](CO[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2N)[C@@H]1O

SVG MW SMILES 3D mol Ionize

R1 = HEP