Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: meningitis [ICD11:
1D01 
];
pneumonia [ICD11:
CA40 ]
The structure was elucidated in this paperNCBI PubMed ID: 20539880Publication DOI: 10.1039/c004090hJournal NLM ID: 101154995Publisher: The Royal Society of Chemistry
Correspondence: annakond
gmail.com
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Russian Federation, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia, Institute of Molecular Genetics, Russian Academy of Sciences, Russian Federation
We established a peculiar structure of the O-specific polysaccharide (O-antigen) of a psychrotrophic strain of Acinetobacter lwoffii, EK30A, isolated from a 1.6-1.8 million-year-old Siberian permafrost subsoil sediment sample. The polysaccharide was released by mild acid degradation of the lipopolysaccharide and studied using chemical analyses, Smith degradation, (1)H and (13)C NMR spectroscopy and mass spectrometry. It was found to contain d-homoserine, which is N-linked to 4-amino-4,6-dideoxy-d-glucose (Qui4N) and is N-acylated itself with acetyl in about half of the repeating units or (S)-3-hydroxybutanoyl group in the other half. The following is the structure of the tetrasaccharide repeating unit of the polysaccharide: →3)-β-D-Quip4NAcyl-(1→6)-α-D-Galp-(1→4)-α-D-GalpNAc-(1→3)-α-D-FucpNAc-(1→ where Acyl stands for either N-acetyl- or N-[(S)-3-hydroxybutanoyl]-d-homoseryl.
structure, O-antigen, identification, Acinetobacter lwoffii, D-homoserine
Structure type: oligomer
Location inside paper: p.3575, fig.6
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_885822
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, Smith degradation, mild acid degradation
Comments, role: the major Smith degradation products. Ac / lS3HOBut ratio at HSer is 50%/50%
Related record ID(s): 25179
NCBI Taxonomy refs (TaxIDs): 28090
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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