Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
];
nosocomial infections [ICD11:
XB25 ];
infection due to Proteus mirabilis [ICD11:
XN9ZF ]
The structure was elucidated in this paperNCBI PubMed ID: 20002745Publication DOI: 10.1111/j.1574-695X.2009.00632.xJournal NLM ID: 9315554Publisher: Elsevier
Correspondence: domkam
biol.uni.lodz.pl
Institutions: Department of General Microbiology, University of Lodz, Lodz, Poland
Seven Proteus mirabilis strains from five Polish patients (five isolates from urea and two from feces) appeared to be a bacterial clone widespread in hospitals, most probably due to nosocomial infection and autoinfection. Enzyme-linked immunosorbent assay and Western blot showed that lipopolysaccharides from all strains studied are serologically identical to each other but distinct from Proteus lipopolysaccharides studied earlier and, hence, these strains could not be classified in any of the currently existing 77 Proteus O-serogroups. Accordingly, structural analysis of the O-polysaccharide of a representative strain 1B-m revealed a structure that is typical of Proteus O-antigens but is unique in detail. Based on these data, we propose to classify the strains studied as a new serogroup in the genus Proteus named O78.
Lipopolysaccharide, Proteus mirabilis, Ribitol phosphate, O-serogroup, nosocomial infection
Structure type: polymer chemical repeating unit
Location inside paper: p.275, fig.5, 3
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_114703,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_1391966,IEDB_141794,IEDB_141807,IEDB_142351,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_591403,IEDB_983931,SB_145,SB_165,SB_166,SB_173,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, HF solvolysis, SDS-PAGE, sugar analysis, GLC, Smith degradation, Western blotting, serological methods
Comments, role: NMR data for Ribitol-5-P-(O- 1H: 3.66-3.86 3.80 3.75 3.93 4.00-4.07; 13C: 63.8 73.6 73.2 72.4 68.1
Related record ID(s): 25234, 25595
NCBI Taxonomy refs (TaxIDs): 584
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,6,0,1 50%Ac 175.8 21.8
3,4,6,0 xDRib-ol 67.0 71.0 72.9 72.5 68.1
3,4,6 P
3,4 bDGalp 104.8 71.2 83.5 69.4 75.0 66.0
3,2,2 Ac 176.2 24.2
3,2 bDGlcpN 103.3 57.2 75.2 71.2 77.4 62.3
3 bDGlcp 102.0 81.0 77.4 81.0 75.9 61.5
2 Ac 175.9 23.7
bDGlcpN 104.3 55.7 84.3 69.6 76.6 62.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,6,0,1 50%Ac - 2.13
3,4,6,0 xDRib-ol 4.20-4.33 4.06 3.80 3.93 4.00-4.07
3,4,6 P
3,4 bDGalp 4.44 3.59 3.72 4.21 3.90 4.06-4.06
3,2,2 Ac - 2.07
3,2 bDGlcpN 4.84 3.75 3.57 3.45 3.42 3.75-3.92
3 bDGlcp 4.62 3.52 3.67 3.61 3.59 3.77-3.93
2 Ac - 2.04
bDGlcpN 4.73 3.82 3.84 3.57 3.46 3.77-3.90
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,6,0,1 50%Ac 21.8/2.13
3,4,6,0 xDRib-ol 67.0/4.20-4.33 71.0/4.06 72.9/3.80 72.5/3.93 68.1/4.00-4.07
3,4,6 P
3,4 bDGalp 104.8/4.44 71.2/3.59 83.5/3.72 69.4/4.21 75.0/3.90 66.0/4.06-4.06
3,2,2 Ac 24.2/2.07
3,2 bDGlcpN 103.3/4.84 57.2/3.75 75.2/3.57 71.2/3.45 77.4/3.42 62.3/3.75-3.92
3 bDGlcp 102.0/4.62 81.0/3.52 77.4/3.67 81.0/3.61 75.9/3.59 61.5/3.77-3.93
2 Ac 23.7/2.04
bDGlcpN 104.3/4.73 55.7/3.82 84.3/3.84 69.6/3.57 76.6/3.46 62.0/3.77-3.90
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,6,0,1 | 50%Ac |
| 2.13 | |
| 3,4,6,0 | xDRib-ol | 4.20
4.33 | 4.06 | 3.80 | 3.93 | 4.00
4.07 | |
| 3,4,6 | P | |
| 3,4 | bDGalp | 4.44 | 3.59 | 3.72 | 4.21 | 3.90 | 4.06
4.06 |
| 3,2,2 | Ac |
| 2.07 | |
| 3,2 | bDGlcpN | 4.84 | 3.75 | 3.57 | 3.45 | 3.42 | 3.75
3.92 |
| 3 | bDGlcp | 4.62 | 3.52 | 3.67 | 3.61 | 3.59 | 3.77
3.93 |
| 2 | Ac |
| 2.04 | |
| | bDGlcpN | 4.73 | 3.82 | 3.84 | 3.57 | 3.46 | 3.77
3.90 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,6,0,1 | 50%Ac | 175.8 | 21.8 | |
| 3,4,6,0 | xDRib-ol | 67.0 | 71.0 | 72.9 | 72.5 | 68.1 | |
| 3,4,6 | P | |
| 3,4 | bDGalp | 104.8 | 71.2 | 83.5 | 69.4 | 75.0 | 66.0 |
| 3,2,2 | Ac | 176.2 | 24.2 | |
| 3,2 | bDGlcpN | 103.3 | 57.2 | 75.2 | 71.2 | 77.4 | 62.3 |
| 3 | bDGlcp | 102.0 | 81.0 | 77.4 | 81.0 | 75.9 | 61.5 |
| 2 | Ac | 175.9 | 23.7 | |
| | bDGlcpN | 104.3 | 55.7 | 84.3 | 69.6 | 76.6 | 62.0 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
