Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 20192967Publication DOI: 10.1111/j.1462-2920.2010.02182.xJournal NLM ID: 100883692Publisher: Blackwell Publishing
Correspondence: jlam
uoguelph.ca
Institutions: Department of Molecular and Cellular Biology, University of Guelph, Ontario N1G 2W1, Canada
Summary The major component of the outer leaflet of the outer membrane of Gram-negative bacteria is lipopolysaccharide (LPS). The outermost domain of LPS is a polysaccharide called O antigen. Pseudomonas aeruginosa establishes biofilms on wet surfaces in a wide range of habitats and mutations in O-antigen biosynthesis genes affect bacterial adhesion and the structure of these biofilms. The P. aeruginosa O6 O antigen contains a 2-acetamido-2-deoxy-d-galacturonamide (d-GalNAcAN) residue. O-antigen biosynthesis in this serotype requires the wbpS gene, which encodes a protein with conserved domains of the glutamine-dependent amidotransferase family. Replacement of conserved amino acids in the N-terminal glutaminase conserved domain of WbpS inhibited O-antigen biosynthesis under restricted-ammonia conditions, but not in rich media; suggesting that this domain functions to provide ammonia for O-antigen biosynthesis under restricted-ammonia conditions, by hydrolysis of glutamine. Escherichia coli O121 also produces a d-GalNAcAN-containing O antigen, and possesses a homologue of wbpS called wbqG. An E. coli O121 wbqG mutant was cross-complemented by providing wbpS in trans, and vice versa, showing that these two genes are functionally interchangeable. The E. coli O121 wbqG mutant O antigen contains 2-acetamido-2-deoxy-d-galacturonate (d-GalNAcA), instead of d-GalNAcAN, demonstrating that wbqG is specifically required for biosynthesis of the carboxamide in this sugar.
biosynthesis, O-antigen, Pseudomonas aeruginosa, Biofilm
Structure type: suggested polymer biological repeating unit
Location inside paper: p.1538, fig.9B
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: 13C NMR, 1H NMR, NMR-2D, X-ray, SDS-PAGE, DNA techniques, Western blotting, genetic methods, microscopy, CE-MS
Biosynthesis and genetic data: genetic data
3D data: 3D data
Related record ID(s): 4019, 20676, 23071, 25302, 25713, 25715, 25716, 25717, 28686, 28687
NCBI Taxonomy refs (TaxIDs): 1055537
Show glycosyltransferases
There is only one chemically distinct structure:
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