Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 20185508Publication DOI: 10.1111/j.1574-695X.2010.00738.xJournal NLM ID: 0376646Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
Correspondence: wanglei
nankai.edu.cn
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin, China, Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, China, Tianjin Research Center for Functional Genomics and Biochip, Tianjin, China, The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, PR China
O-antigen is a component of the outer membrane of Gram-negative bacteria and is one of the most variable cell surface constituents, leading to major antigenic variability. The O-antigen forms the basis for bacterial serotyping. In this study, the O-antigen structure of Salmonella O66 was established, which differs from the known O-antigen structure of Escherichia coli O166 only in one linkage (most likely the linkage between the O-units) and O-acetylation. The O-antigen gene clusters of Salmonella O66 and E. coli O166 were found to have similar organizations, the only exception being that in Salmonella O66, the wzy gene is replaced by a non-coding region. The function of the wzy gene in E. coli O166 was confirmed by the construction and analysis of deletion and trans-complementation mutants. It is proposed that a functional wzy gene located outside the O-antigen gene cluster is involved in Salmonella O66 O-antigen biosynthesis, as has been reported previously in Salmonella serogroups A, B and D1. The sequence identity for the corresponding genes between the O-antigen gene clusters of Salmonella O66 and E. coli O166 ranges from 64 to 70 %, indicating that they may originate from a common ancestor. It is likely that after the species divergence, Salmonella O66 got its specific O-antigen form by inactivation of the wzy gene located in the O-antigen gene cluster and acquisition of two new genes (a wzy gene and a prophage gene for O-acetyl modification) both residing outside the O-antigen gene cluster.
structure, O-antigen, Escherichia, O-acetylation, Salmonella enterica, Escherichia coli O166, Salmonella O66, O-antigen gene clusters
Structure type: suggested polymer biological repeating unit
Location inside paper: p.1646, fig.2, 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_134624,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_141582,IEDB_141584,IEDB_141794,IEDB_142488,IEDB_144989,IEDB_146664,IEDB_151528,IEDB_153207,IEDB_153208,IEDB_190606,IEDB_885822,IEDB_983931,SB_163,SB_192,SB_21,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, GLC, de-O-acetylation, NMR-1D, genetic methods
Biosynthesis and genetic data: genetic data
Related record ID(s): 21628, 25329, 30394
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G70929JC, GlycomeDB:
34695
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error