Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 20110088Publication DOI: 10.1016/j.carres.2009.11.026Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M.B. Perry <malcolm.perry
nrc-cnrc.gc.ca>
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6
The structure of the antigenic O-polysaccharide (O-PS) produced by Escherichia coli serotype O:70 was determined by analysis of the chromatographically purified O-PS polymer prepared by mild hydrolysis of its aqueous phenol-extracted smooth-type somatic lipopolysaccharide. The O-PS is composed of d-glucose, d-galactose, d-fucose, 2-acetamido-2-deoxy-d-galactose, and 3-acetamido-3-deoxy-d-quinovose in a ratio of 1:1:1:1:1. From the use of DOC-PAGE, methylation, Smith-type periodate oxidation, and (1)H and (13)C NMR spectroscopy, including 2D experiments, the O-PS was shown to be a polymer of a branched repeating pentasaccharide unit having the structure: (formula, see text).
Lipopolysaccharide, antigen, structure, polysaccharide, Escherichia coli O:70
Structure type: polymer chemical repeating unit
Location inside paper: abstrat, p.646, p.647, scheme 1 (polysaccharide II)
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115015,IEDB_130648,IEDB_134627,IEDB_136044,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_141584,IEDB_141794,IEDB_142489,IEDB_143260,IEDB_149135,IEDB_190606,IEDB_885822,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_7,SB_8,SB_86,SB_88
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, GLC, DOC-PAGE, Smith degradation
Comments, role: O-polysaccharide after Smith degradation
Related record ID(s): 25335
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G64830JE, GlycomeDB:
37887
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,3 bDGalp 106.0 70.3 78.9 66.4 75.8 62.4
3,4,2 Ac
3,4 aDGalpN 98.5 49.1 78.6 69.8 72.4 62.4
3,3 Ac
3 bDQuip3N 104.5 73.8 57.9 77.5 73.3 19.5
aDFucp 96.8 68.3 80.0 72.9 67.8 16.4
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3 bDGalp 4.49 3.62 3.66 4.12 3.64 3.77
3,4,2 Ac
3,4 aDGalpN 5.24 4.38 3.92 4.29 4.10 3.64-3.75
3,3 Ac
3 bDQuip3N 4.75 3.34 4.07 3.52 3.66 1.36
aDFucp 5.07 3.99 4.12 4.02 4.34 1.20
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,3 bDGalp 106.0/4.49 70.3/3.62 78.9/3.66 66.4/4.12 75.8/3.64 62.4/3.77
3,4,2 Ac
3,4 aDGalpN 98.5/5.24 49.1/4.38 78.6/3.92 69.8/4.29 72.4/4.10 62.4/3.64-3.75
3,3 Ac
3 bDQuip3N 104.5/4.75 73.8/3.34 57.9/4.07 77.5/3.52 73.3/3.66 19.5/1.36
aDFucp 96.8/5.07 68.3/3.99 80.0/4.12 72.9/4.02 67.8/4.34 16.4/1.20
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3 | bDGalp | 4.49 | 3.62 | 3.66 | 4.12 | 3.64 | 3.77 |
| 3,4,2 | Ac | |
| 3,4 | aDGalpN | 5.24 | 4.38 | 3.92 | 4.29 | 4.10 | 3.64
3.75 |
| 3,3 | Ac | |
| 3 | bDQuip3N | 4.75 | 3.34 | 4.07 | 3.52 | 3.66 | 1.36 |
| | aDFucp | 5.07 | 3.99 | 4.12 | 4.02 | 4.34 | 1.20 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,3 | bDGalp | 106.0 | 70.3 | 78.9 | 66.4 | 75.8 | 62.4 |
| 3,4,2 | Ac | |
| 3,4 | aDGalpN | 98.5 | 49.1 | 78.6 | 69.8 | 72.4 | 62.4 |
| 3,3 | Ac | |
| 3 | bDQuip3N | 104.5 | 73.8 | 57.9 | 77.5 | 73.3 | 19.5 |
| | aDFucp | 96.8 | 68.3 | 80.0 | 72.9 | 67.8 | 16.4 |
|
There is only one chemically distinct structure:
report error
Found 1 record.
Displayed record 1
