Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 21044779Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M.B. Perry <malcolm.perry
nrc-cnrc.gc.ca>
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A 0R6
Mild acid hydrolysis of the lipopolysaccharide produced by Escherichiacoli O118:H16 standard strain (NRCC 6613) afforded an O-polysaccharide (O-PS) composed of d-galactose, 2-acetamidoylamino-2,6-dideoxy-L-galactose, 2-acetamido-2-deoxy-D-glucose, ribitol, and phosphate (1:1:1:1:1). From DOC-PAGE, sugar and methylation analyses, one- and two-dimensional NMR spectroscopy, capillary electrophoresis-mass spectrometry, hydrolysis, and sequential Smith-type periodate oxidation studies, the O-PS was determined to be an unbranched linear polymer having the structure: [6)-α-D-Galp-(1→3)-α-L-FucpNAm-(1→3)-β-D-GlcpNAc-(1→3)-Rib-ol-5-P-(O→](n) The structure of the O-PS is consistent with the reported DNA data on the O-antigen gene-cluster of E. coli O118 and interestingly, the O-PS is similar to the structures of the O-antigens of Salmonellaenterica O47 and E. coli O151:H10 reference strain 880-67, as predicted from the results of DNA sequencing of their respective O-antigen gene-clusters
Lipopolysaccharide, NMR, antigen, structure, DNA, strain, structural, characterization, polysaccharide, serotype, O-antigen, O antigen, polymer, Research, Escherichia coli, acid, phosphate, NMR spectroscopy, gene cluster, O-polysaccharide, O antigens, O polysaccharide, O-antigens, hydrolysis, spectrometry, biological, reference, sugar, DNA sequencing, methylation, sequencing, spectroscopy, ribitol, linear, oxidation, standard, D-galactose, 2-acetamido-2-deoxy-D-glucose, sequential, Periodate oxidation, periodate
Structure type: oligomer
Location inside paper: abstract, p.2669, scheme 1, O-PS(Mod) IIa
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, HF solvolysis, sugar analysis, 31P NMR, ESI-MS, GLC, mild acid hydrolysis, DOC-PAGE, Smith degradation, NMR-1D, CE-MS
Comments, role: modified O-polysaccharide after HF solvolysis and Smith degradation
Related record ID(s): 25336, 25752, 25753, 25754
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G63010IG
Show glycosyltransferases
There is only one chemically distinct structure:
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