Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 20555385Journal NLM ID: 8606068Publisher: Ottawa: National Research Council of Canada
Correspondence: M.B. Perry <malcolm.perry
nrc-cnrc.gc.ca>
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON K1A 0R6, Canada
The antigenic O-polysaccharide component of the lipopolysaccharide produced by Escherichia coli serotype O71:H12 was analyzed by chemical composition, nuclear magnetic spectroscopy, and Smith-type periodate oxidation methods. It was determined to be a partially O-acetylated unbranched polymer of a repeating tetrasaccharide unit composed of L-rhamnose, D-galactose, 2-acetamido-2-deoxy-D-galactose, and 3-acetamido-3-deoxy-D-quinovose (1:1:1:1) residues having the following structure: [structure: see text].
Lipopolysaccharide, polysaccharide, O-antigen, Escherichia coli O71
Structure type: oligomer
Location inside paper: p.442, scheme 1, [I]
Contained glycoepitopes: IEDB_114709,IEDB_130648,IEDB_136105,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_225177,IEDB_885822,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, DOC-PAGE, Smith degradation, de-O-acetylation, NMR-1D
Comments, role: product of first Smith degradation of the O-deacylated O-PS
Related record ID(s): 25338, 25758, 25760
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GlycomeDB:
38079
Show glycosyltransferases
NMR conditions: in D2O at 278 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,3,4,2 Ac
2,3,4 aDGalpN 98.1 50.5 68.5 69.6 72.8 62.4
2,3,3 Ac
2,3 aDQuip3N 96.1 71.6 55.1 77.3 68.4 19.2
2 aLRhap 100 68.6 77.3 71.4 70.5 17.9
xDThre-ol 61.2 77.3 72.5 63.8
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,3,4,2 Ac
2,3,4 aDGalpN 5.27 4.18 3.85 4.00 4.06 3.75
2,3,3 Ac
2,3 aDQuip3N 5.03 3.57 4.31 3.52 4.22 1.31
2 aLRhap 5.05 4.19 3.86 3.62 3.86 1.33
xDThre-ol 3.71-3.84 3.80 3.87 3.65-3.72
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,3,4,2 Ac
2,3,4 aDGalpN 98.1/5.27 50.5/4.18 68.5/3.85 69.6/4.00 72.8/4.06 62.4/3.75
2,3,3 Ac
2,3 aDQuip3N 96.1/5.03 71.6/3.57 55.1/4.31 77.3/3.52 68.4/4.22 19.2/1.31
2 aLRhap 100/5.05 68.6/4.19 77.3/3.86 71.4/3.62 70.5/3.86 17.9/1.33
xDThre-ol 61.2/3.71-3.84 77.3/3.80 72.5/3.87 63.8/3.65-3.72
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,3,4,2 | Ac | |
| 2,3,4 | aDGalpN | 5.27 | 4.18 | 3.85 | 4.00 | 4.06 | 3.75 |
| 2,3,3 | Ac | |
| 2,3 | aDQuip3N | 5.03 | 3.57 | 4.31 | 3.52 | 4.22 | 1.31 |
| 2 | aLRhap | 5.05 | 4.19 | 3.86 | 3.62 | 3.86 | 1.33 |
| | xDThre-ol | 3.71
3.84 | 3.80 | 3.87 | 3.65
3.72 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,3,4,2 | Ac | |
| 2,3,4 | aDGalpN | 98.1 | 50.5 | 68.5 | 69.6 | 72.8 | 62.4 |
| 2,3,3 | Ac | |
| 2,3 | aDQuip3N | 96.1 | 71.6 | 55.1 | 77.3 | 68.4 | 19.2 |
| 2 | aLRhap | 100 | 68.6 | 77.3 | 71.4 | 70.5 | 17.9 |
| | xDThre-ol | 61.2 | 77.3 | 72.5 | 63.8 | |
|
There is only one chemically distinct structure:
Found 
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