Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 20555385Journal NLM ID: 8606068Publisher: Ottawa: National Research Council of Canada
Correspondence: M.B. Perry <malcolm.perry
nrc-cnrc.gc.ca>
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON K1A 0R6, Canada
The antigenic O-polysaccharide component of the lipopolysaccharide produced by Escherichia coli serotype O71:H12 was analyzed by chemical composition, nuclear magnetic spectroscopy, and Smith-type periodate oxidation methods. It was determined to be a partially O-acetylated unbranched polymer of a repeating tetrasaccharide unit composed of L-rhamnose, D-galactose, 2-acetamido-2-deoxy-D-galactose, and 3-acetamido-3-deoxy-D-quinovose (1:1:1:1) residues having the following structure: [structure: see text].
Lipopolysaccharide, polysaccharide, O-antigen, Escherichia coli O71
Structure type: oligomer
Location inside paper: p.442, scheme 1, [II]
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, DOC-PAGE, Smith degradation, de-O-acetylation, NMR-1D
Comments, role: product of second Smith degradation of the O-deacylated O-PS
Related record ID(s): 25338, 25758, 25759
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G57791AR
Show glycosyltransferases
NMR conditions: in D2O at 278 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,3,3 Ac
2,3 aDQuip3N 95.2 70.2 54.0 73.4 68.4 16.7
2 aLRhap 99.1 67.4 76.1 70.3 69.1 16.8
xDGro 60.3 78.4 61.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,3,3 Ac
2,3 aDQuip3N 5.04 3.63 4.12 3.19 4.14 1.25
2 aLRhap 5.02 4.19 3.88 3.61 3.91 1.33
xDGro 3.7-3.8 3.81 3.72
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,3,3 Ac
2,3 aDQuip3N 95.2/5.04 70.2/3.63 54.0/4.12 73.4/3.19 68.4/4.14 16.7/1.25
2 aLRhap 99.1/5.02 67.4/4.19 76.1/3.88 70.3/3.61 69.1/3.91 16.8/1.33
xDGro 60.3/3.7-3.8 78.4/3.81 61.5/3.72
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,3,3 | Ac | |
| 2,3 | aDQuip3N | 5.04 | 3.63 | 4.12 | 3.19 | 4.14 | 1.25 |
| 2 | aLRhap | 5.02 | 4.19 | 3.88 | 3.61 | 3.91 | 1.33 |
| | xDGro | 3.7
3.8 | 3.81 | 3.72 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,3,3 | Ac | |
| 2,3 | aDQuip3N | 95.2 | 70.2 | 54.0 | 73.4 | 68.4 | 16.7 |
| 2 | aLRhap | 99.1 | 67.4 | 76.1 | 70.3 | 69.1 | 16.8 |
| | xDGro | 60.3 | 78.4 | 61.5 | |
|
There is only one chemically distinct structure:
Found 
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