CSDB: Search results

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: tularemia [ICD11: 1B94

, ICD11: XN0BX

]; infection due to Francisella tularensis [ICD11: XN0BX

]

The structure was elucidated in this paper
NCBI PubMed ID: 20618133
Journal NLM ID: 0376536
Publisher: Nauka/Interperiodica
Correspondence: mokrievich

obolensk.org
Institutions: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia

A knockout mutant with a deletion in a quorum sensing system gene qseC was generated from the vaccine strain Francisella tularensis 15 by site-directed mutagenesis. The variant with the inactivated gene qseC differed from the parental strain in growth rate on solid nutrient medium but had the same growth dynamics in liquid nutrient medium. The mutation abolished almost completely the resistance of the vaccine strain to normal rabbit serum and its ability to survive in macrophages; in addition, the strain lost the residual virulence. A significant phenotypic alteration was observed in the lipopolysaccharide of F. tularensis. Particularly, the mutant strain synthesized no noticeable amount of the lipopolysaccharide with the high-molecular-mass O-polysaccharide, presumably as a result of impairing biosynthesis of the repeating unit, namely, a loss of the ability to incorporate a formyl group, an N-acyl substituent of 4-amino-4,6-dideoxy-D-glucose.

Lipopolysaccharide, Francisella tularensis, tularemia, quorum sensing, gene qseC, sitedirected mutagenesis

Structure type: oligomer
Location inside paper: p.448, fig.3, (3)
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_130701,IEDB_137473,IEDB_137485,IEDB_1391961,IEDB_141584,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_153219,IEDB_244149,IEDB_885822,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72

Methods: SDS-PAGE, DNA techniques, mild acid hydrolysis, ESI-ICR-MS, Western blotting, genetic methods
Biological activity: phagocytosis, virulence, biological properties
Biosynthesis and genetic data: genetic data
Comments, role: the major glycoform OS1 of the SR-form F.tularensis 15 (qsecC)

Related record ID(s): 25351, 25780, 25782
NCBI Taxonomy refs (TaxIDs): 263
Reference(s) to other database(s): GTC:G38763OW
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@H]1[C@@H](O[C@H]2[C@@H](C(N)=O)O[C@H](O[C@@H]3[C@@H](NC(C)=O)[C@H](O[C@H]4[C@H](O)[C@H](O[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5NC(C)=O)[C@H](O[C@H]5[C@H](O[C@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@H](O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)[C@@H](O[C@@H]6[C@H](O)C[C@](O)(C(=O)O)O[C@@H]6[C@H](O)CO)O[C@@H]5CO)O[C@@H]4CO)O[C@H](C)[C@H]3O)[C@H](NC(C)=O)[C@H]2O)O[C@H](C(N)=O)[C@H](O[C@@H]2O[C@H](C)[C@@H](N)[C@H](O)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize