Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Citrobacter [ICD11:
XN0FZ 
]
The structure was elucidated in this paperNCBI PubMed ID: 10542050Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: Lennart.Kenne
kemi.slu.se
Institutions: Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden, Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland
The structure of the O-specific side-chain of the Hafnia alvei strain PCM1207 lipopolysaccharide (LPS) has been investigated. Methylation analysis, partial acid hydrolysis, matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) MS, fast atom bombardment (FAB)-MS/MS and 1H- and 13C NMR spectroscopy were the principal methods used. Glycerol phosphate was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was established: -3)[aDGlcp(1-6),Ac(1-2)]bDGalpN(1-4)[xDGro(1-P-3),Ac(1-2)]bDGalpN(1-3)aDGalp(1-4)bDGalp(1-. The polysaccharide is partially (< 10%) substituted with O-acetyl groups. The lipopolysaccharide was also subjected to high resolution magic angle spinning (HR-MAS) NMR analysis, which showed both the signals of the O-specific polysaccharide as well as several signals from unsubstituted core oligosaccharides. This confirmed the presence of the described structure in the native LPS.
Lipopolysaccharide, O-antigen, Hafnia alvei, MALDI-TOF, MALDI-TOF MS, HR-MAS NMR
Structure type: polymer chemical repeating unit
Location inside paper: page 60
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130695,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_167069,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: methylation, NMR-2D, partial acid hydrolysis, ELISA, MALDI-TOF MS, de-O-acetylation, FAB-MS/MS, TEMPO oxidation
Biological activity: serological data
Comments, role: absolute configuration of glycerol was determined
Related record ID(s): 2475, 2585
NCBI Taxonomy refs (TaxIDs): 544Reference(s) to other database(s): GTC:G62463SC
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There is only one chemically distinct structure:
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