Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: pertussis (whooping cough) [ICD11:
1C12.Z 
, ICD11:
XN9W3 ];
respiratory infections [ICD11:
CA45 ];
infection due to Bordetella bronchiseptica [ICD11:
XN173 ]
NCBI PubMed ID: 20592026Publication DOI: 10.1074/jbc.M110.115121Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: a.preston
bristol.ac.uk
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada
The O chain polysaccharide (O PS) of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharide is a homopolymer of 2,3-diacetamido-2,3-dideoxygalacturonic acid (GalNAc3NAcA) in which some of the sugars are present as uronamides. The terminal residue contains several unusual modifications. To date, two types of modification have been characterized, and a survey of numerous strains demonstrated that each contained one of these two modification types. Host antibody responses against the O PS are directed against the terminal residue modifications, and there is little cross-reactivity between the two types. This suggests that Bordetella O PS modifications represent a means of antigenic variation. Here we report the characterization of the O PS of B. bronchiseptica strain MO149. It consists of a novel two-sugar repeating unit and a novel terminal residue modification, with the structure Me-4-α-L-GalNAc3NAcA-(4-β-D-GlcNAc3NAcA-4-α-L-GalNAc3NAcA-)(5-6)-, which we propose be defined as the B. bronchiseptica O3 PS. We show that the O3 PS is very poorly immunogenic and that the MO149 strain contains a novel wbm (O PS biosynthesis) locus. Thus, there is greater diversity among Bordetella O PSs than previously recognized, which is likely to be a result of selection pressure from host immunity. We also determine experimentally, for the first time, the absolute configuration of the diacetimido-uronic acid sugars in Bordetella O PS.
O polysaccharide, bacteria, Bordetella, immunity, lipopolysaccharide (LPS), evolution, Bacterial Genetics, Humoral Response
Structure type: oligomer
Location inside paper: p.26871, fig.1
Aglycon: linker+core
Compound class: O-polysaccharide
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, HF solvolysis, sugar analysis, ESI-MS, mild acid hydrolysis, Western blotting, serological methods, genetic methods
Biosynthesis and genetic data: genetic data
Comments, role: part of structure (see RR: 25877,25880); revised
Related record ID(s): 25876, 25877, 25878, 25880
NCBI Taxonomy refs (TaxIDs): 518
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error