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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: pertussis (whooping cough) [ICD11: 1C12.Z

, ICD11: XN9W3

]; respiratory infections [ICD11: CA45

]; infection due to Bordetella bronchiseptica [ICD11: XN173

]

NCBI PubMed ID: 20592026
Publication DOI: 10.1074/jbc.M110.115121
Journal NLM ID: 2985121R
Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: a.preston

bristol.ac.uk
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada

The O chain polysaccharide (O PS) of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharide is a homopolymer of 2,3-diacetamido-2,3-dideoxygalacturonic acid (GalNAc3NAcA) in which some of the sugars are present as uronamides. The terminal residue contains several unusual modifications. To date, two types of modification have been characterized, and a survey of numerous strains demonstrated that each contained one of these two modification types. Host antibody responses against the O PS are directed against the terminal residue modifications, and there is little cross-reactivity between the two types. This suggests that Bordetella O PS modifications represent a means of antigenic variation. Here we report the characterization of the O PS of B. bronchiseptica strain MO149. It consists of a novel two-sugar repeating unit and a novel terminal residue modification, with the structure Me-4-α-L-GalNAc3NAcA-(4-β-D-GlcNAc3NAcA-4-α-L-GalNAc3NAcA-)(5-6)-, which we propose be defined as the B. bronchiseptica O3 PS. We show that the O3 PS is very poorly immunogenic and that the MO149 strain contains a novel wbm (O PS biosynthesis) locus. Thus, there is greater diversity among Bordetella O PSs than previously recognized, which is likely to be a result of selection pressure from host immunity. We also determine experimentally, for the first time, the absolute configuration of the diacetimido-uronic acid sugars in Bordetella O PS.

O polysaccharide, bacteria, Bordetella, immunity, lipopolysaccharide (LPS), evolution, Bacterial Genetics, Humoral Response

Structure type: oligomer
Location inside paper: p.26871, fig.1
Aglycon: lipid A
Trivial name: linker+core
Compound class: LPS
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_140630,IEDB_141584,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_2189047,IEDB_2275071,IEDB_885822,IEDB_983931,SB_192

Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, HF solvolysis, sugar analysis, ESI-MS, mild acid hydrolysis, Western blotting, serological methods, genetic methods
Biosynthesis and genetic data: genetic data
Comments, role: part of structure: linker+core (see ID 25876,25878,25879)

Related record ID(s): 25876, 25877, 25878, 25879
NCBI Taxonomy refs (TaxIDs): 1208658, 518
Show glycosyltransferases

There are 3 chemically distinct structures. Please, select:

[1*]O[C@H]1O[C@H]([C@@H](O)CO)[C@@H](O[C@@H]2O[C@H](CO[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3N)[C@@H](O[C@H]3O[C@H](CO[C@@H]4O[C@H](C)[C@H](NC)[C@H](O[C@@H]5O[C@H](C(=O)O)[C@@H](O[C@H]6O[C@H](CO[C@@H]7O[C@H](C)[C@H](NC)[C@H](O[C@@H]8O[C@H](C(=O)O)[C@@H](O[C@H]9O[C@H](CO)[C@H](O[C@@H]%10O[C@H](C(N)=O)[C@@H](O[C@@H]%11O[C@H](C(N)=O)[C@@H](O[2*])[C@H](NC(C)=O)[C@@H]%11NC(C)=O)[C@H](NC(C)=O)[C@H]%10NC(C)=O)[C@H](O)[C@H]9NC(C)=O)[C@H](NC(C)=O)[C@@H]8NC(C)=O)[C@H]7NC(C)=O)[C@@H](O)[C@H](O)[C@H]6NC(C)=O)[C@H](NC(C)=O)[C@@H]5NC(C)=O)[C@H]4NC(C)=O)[C@@H](O[C@H]4O[C@H]([C@@H](O)CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@H](O)[C@H]3N)[C@H](O)[C@H]2O)[C@H](O[C@H]2O[C@H]([C@@H](O)CO[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3N)[C@@H](O)[C@H](OP(=O)(O)O)[C@@H]2O[C@H]2O[C@H](C(=O)O)[C@@H](O)[C@H](O)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

R1 = ??Sug?, Sug = anhKdo
R2 = O-antigen (ID 25876, 25878, 25879)