Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: necrotising pneumonia [ICD11:
CA40 
, ICD11:
CA43.0 , ICD11:
XN3PW ]
The structure was elucidated in this paperNCBI PubMed ID: 20409536Publication DOI: 10.1016/j.carres.2010.03.029Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: P.Cescutti <pcescutti
units.it>
Institutions: Department of Life Sciences, University of Trieste, Via L. Giorgieri 1, Bdg C11, I-34127 Trieste, Italy
The repeating unit of cepacian, the exopolysaccharide produced by the majority of the microorganisms belonging to the Burkholderia cepacia complex, was isolated from inner bacterial membranes and investigated by mass spectrometry, with and without prior derivatisation. Interpretation of the mass spectra led to the determination of the biological repeating unit primary structure, thus disclosing the nature of the oligosaccharide produced in vivo. Moreover, mass spectra recorded on the native sample revealed that acetyl substitution was very variable, producing a mixture of repeating units containing zero to four acyl groups. At the same time, finding acetylated oligosaccharides showed that binding of these substituents occurred in the cellular periplasmic space, before the polymerisation process took place. In the chromatographic peak containing the repeating unit, oligosaccharides shorter than the repeating unit co-eluted. Mass spectrometric analysis showed that they were biosynthetic intermediates of the repeating unit and further investigation revealed the biosynthetic sequence of cepacian building block.
structure, polysaccharide, repeating unit, ESI-MS, Burkholderia cepacia complex
Structure type: polymer chemical repeating unit
Location inside paper: p.1455, fig.1
Trivial name: cepacian
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_1394181,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88
Methods: ESI-MS, acid hydrolysis, permethylation
Related record ID(s): 1207, 3154, 4338, 7433, 10142, 10158, 10790, 20223, 21560, 22655, 26105
NCBI Taxonomy refs (TaxIDs): 292Reference(s) to other database(s): GTC:G16935RB, GlycomeDB:
25352
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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(previously named: Pseudomonas cepacia)