Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Cyprinus carpio
Associated disease: motile aeromonad septicemia (MAS)
NCBI PubMed ID: 37437416Publication DOI: 10.1016/j.carres.2023.108896Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: A. Turska-Szewczuk <anna.turska-szewczuk
mail.umcs.pl>
Institutions: Department of Genetics and Microbiology, Institute of Biological Sciences, M. Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland, Department of Omics Analyses, National Veterinary Research Institute, Partyzantow 57, 24-100, Pulawy, Poland, Laboratory of General Microbiology, Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237, Lodz, Poland, Department of Preclinical Sciences and Infectious Diseases, Faculty of Veterinary Medicine and Animal Science, Poznan University of Life Sciences, Wolynska 35, 60-637, Poznan, Poland
The present study included three Aeromonas sp. strains isolated from fish tissues during Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreaks on commercial farms, i.e.: Aeromonas hydrophila Pt679 obtained from rainbow trout as well as Aeromonas popoffii A4 (formerly Aeromonas encheleia) and Aeromonas sobria K928 both isolated from carp, which were classified into the new provisional PGO1 serogroup prevailing among aeromonads in Polish aquaculture. The structure of the O-specific polysaccharides of A4 and K928 has been previously established. Here, immunochemical studies of the O-specific polysaccharide of A. hydrophila Pt679 were undertaken. The O-specific polysaccharide was obtained from the lipopolysaccharide of A. hydrophila Pt679 after mild acid hydrolysis and separation by gel-permeation chromatography. The high-molecular-mass fraction was studied using chemical methods and 1H and 13C NMR spectroscopy, including 1H,1H NOESY, and 1H,13C HMBC experiments. The following structure of the branched repeating unit of the O-polysaccharide from A. hydrophila Pt679 was determined: [Formula: see text] The studies indicated that O-polysaccharides from A. hydrophila Pt679, A. popoffii A4 and A. sobria K928 share similarities but they also contain unique characteristics. Western blotting and an enzyme-linked immunosorbent assay revealed that the cross-reactivity of the related O-antigens is caused by the occurrence of common structural elements, whereas additional epitopes define the specificity of the O-serotypes. For genetic relationship studies, the O-antigen gene cluster was characterized in the genome of the A. hydrophila Pt679 strain and compared with the corresponding sequences of A. popoffii A4 and A. sobria K928 and with sequences available in the databases. The composition of the regions was found to be consistent with the O-antigen structures of Aeromonas strains classified into the same PGO1 serogroup.
NMR, O-antigen, O-antigen gene cluster, Aeromonas, QuiNAc, Fucp3NAc
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 7, A. sobria PGO1a K928
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, SDS-PAGE, sugar analysis, DNA techniques, ELISA, mild acid hydrolysis, Western blotting, GPC, function analysis of gene clusters
Related record ID(s): 22267, 25950
NCBI Taxonomy refs (TaxIDs): 646
Show glycosyltransferases
There is only one chemically distinct structure:
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