Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 37374879Publication DOI: 10.3390/microorganisms11061377Journal NLM ID: 101625893Publisher: Basel, Switzerland: MDPI AG
Correspondence: G. Koudelka <koudelka
buffalo.edu>
Institutions: Department of Biological Sciences, University at Buffalo, Buffalo, NY 14260, USA
Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment. To enhance their survival, bacteria evolved many defense strategies to avoid grazing by protists. Cell wall modification is one of the defense strategies that helps bacteria escape from recognition and/or internalization by its predators. Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall. LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide. O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown. Here, we investigate how length, structure and composition of LPS affect E. coli recognition and internalization by A. castellanii. We found that length of O-antigen does not play a significant role in regulating bacterial recognition by A. castellanii. However, the composition and structure of O-polysaccharide play important roles in providing resistance to A. castellanii predation.
Lipopolysaccharide, O-polysaccharide, A. castellanii, protozoan predation
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 5(a), O98 unit
Aglycon: core-lipid A
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531
Methods: PCR, SDS-PAGE, genetic methods, statistical analysis, competition assays, cultivation, predation assay
Related record ID(s): 22273, 25952, 25953, 25954, 25955, 25956, 25957, 25958, 25960
NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases
There is only one chemically distinct structure:
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