Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Homo sapiens
Associated disease: infection due to Bacillus subtilis [ICD11:
XM4SG9 
];
infection due to Staphylococcus epidermidis [ICD11:
XN8KJ ]
NCBI PubMed ID: 20400947Publication DOI: 10.1038/nsmb.1819Journal NLM ID: 101186374Publisher: Nature Publishing Group
Correspondence: natalie
byron.biochem.ubc.ca
Institutions: Department of Biochemistry and Molecular Biology, Vancouver, Canada
Teichoic acid polymers are composed of polyol-phosphate units and form a major component of Gram-positive bacterial cell walls. These anionic compounds perform a multitude of important roles in bacteria and are synthesized by monotopic membrane proteins of the TagF polymerase family. We have determined the structure of Staphylococcus epidermidis TagF to 2.7-A resolution from a construct that includes both the membrane-targeting region and the glycerol-phosphate polymerase domains. TagF possesses a helical region for interaction with the lipid bilayer, placing the active site at a suitable distance for access to the membrane-bound substrate. Characterization of active-site residue variants and analysis of a CDP-glycerol substrate complex suggest a mechanism for polymer synthesis. With the importance of teichoic acid in Gram-positive physiology, this elucidation of the molecular details of TagF function provides a critical new target in the development of novel anti-infectives.
synthesis, structure, cell wall, Staphylococcus, teichoic acid, substrate, Gram-positive, Staphylococcus epidermidis, polymerase TagF
Structure type: oligomer
Location inside paper: p.583, fig.1
Aglycon: peptidoglycan
Compound class: teichoic acid
Contained glycoepitopes: IEDB_130695,IEDB_137340,IEDB_141807,IEDB_150077,IEDB_151531,IEDB_1597446,IEDB_885813
Methods: genetic methods, crystallography, statistical analysis
Biosynthesis and genetic data: genetic data
3D data: 3D data
NCBI Taxonomy refs (TaxIDs): 1423,
1282
Show glycosyltransferases
There is only one chemically distinct structure:
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