Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: infection due to Bordetella pertussis [ICD11:
XN23B 
]
The structure was elucidated in this paperNCBI PubMed ID: 21050116Publication DOI: 10.1086/657409Journal NLM ID: 0413675Publisher: Oxford: Oxford University Press
Correspondence: rachelf
interchange.ubc.ca
Institutions: Department of Microbiology & Immunology, University of British Columbia, Vancouver, British Columbia, Canada, Equipe Structure et Activités des Endotoxines, UMR 8621 du Centre National de la Recherche Scientifique, IGM, Université de Paris-Sud, Orsay, France, Department of Comparative Medicine, University of Washington, Seattle, Washington
Bordetella endotoxins show remarkable structural variability both among each other and in comparison to other gram-negative bacteria. Here we demonstrate that, in contrast to the common Bordetella pertussis laboratory strain and Tohama I derivative BP338, lipooligosaccharide from mouse challenge strain 18-323 is a poor inducer of inflammatory cytokines in human and murine macrophages, is greatly impaired in Toll-like receptor 4-mediated activation of nuclear factor-kappaB in transfected HEK-293 cells, and functions as a Toll-like receptor 4 antagonist. Comparison of lipid A and lipooligosaccharide structures of B. pertussis strains BP338 and 18-323 revealed that 18-323 (1) lacks the ability to modify its lipid A phosphate groups with glucosamine, (2) is distinct in its acylation at the C3' position of the lipid A diglucosamine backbone, and (3) expresses molecular lipooligosaccharide species that lack a terminal heptose. Our findings have important implications for interpreting previous studies of host defenses to B. pertussis infection in mice and in vitro.
lipopolysaccharides, Lipooligosaccharide, lipid A, Bordetella pertussis, gram negative bacteria, cytokines, macrophage, NF-kappa B, endotoxicity
Structure type: oligomer ; 1559 [M-H]-, 1720 [M-H]-, 1881 [M-H]-
Location inside paper: p.1904, fig.6E
Compound class: lipid A
Contained glycoepitopes: IEDB_141807,IEDB_151531,IEDB_176772
Methods: GC, MALDI-MS, statistical analysis
Biological activity: endotoxin activity data
Related record ID(s): 25928, 25929
NCBI Taxonomy refs (TaxIDs): 520
Show glycosyltransferases
There is only one chemically distinct structure:
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