Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 20717972Journal NLM ID: 7502021Publisher: New York: Wiley-VCH
Correspondence: dordick
rpi.edu
Institutions: Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioengineered heparin. Heparosan is also the capsular polysaccharide of Escherichia coli K5 strain. The current study was focused on the examination of the fermentation of E. coli K5 with the goal of producing heparosan in high yield and volumetric productivity. The structure and molecular weight properties of this bacterial heparosan were determined using polyacrylamide gel electrophoresis (PAGE) and Fourier transform mass spectrometry. Fermentation of E. coli K5 in a defined medium using exponential fed-batch glucose addition with oxygen enrichment afforded heparosan at 15 g/L having a number average molecular weight of 58,000 Da and a weight average molecular weight of 84,000 Da.
polysaccharide, capsule, heparin, E. coli, Heparosan, ermentation
Structure type: polymer chemical repeating unit ; n=24, 84000
Location inside paper: p.965, fig.1A, p.968
Trivial name: heparosan
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_151531,IEDB_153764,IEDB_423153
Methods: 13C NMR, 1H NMR, ESI-MS, fermentation, HPLC-MS, FT-MS
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G26089XS
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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