Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Oryctolagus cuniculus; Homo sapiens
Associated disease: respiratory infections [ICD11:
CA45 
];
infection due to Bordetella bronchiseptica [ICD11:
XN173 ]
The structure was elucidated in this paperNCBI PubMed ID: 21452385Journal NLM ID: 8802365Publisher: John Wiley And Sons Ltd
Correspondence: martine.caroff
u-psud.fr
Institutions: Endotoxines, Structures et Activités, (ESA) UMR 8621, GDR 3048, du CNRS, Institut de Génétique et Microbiologie, Université de Paris-Sud, Orsay, France, Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, 75724 Paris Cedex 15, France, CNRS URA3012, GDR3048
Bordetella bronchiseptica is a respiratory pathogen in mammal species and its cell surface lipopolysaccharide-endotoxin is a potent virulence factor. In order to better characterize the endotoxin structure to virulence relationships, we studied the lipid A structures of B. bronchiseptica isolates from human and rabbit origins as a function of their virulence phases. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been widely used for the structural characterization of bacterial endotoxins and their lipid A moieties. This method combined with chemical analytical methods proved to be essential for the characterization of small samples and discrete but essential structural modifications. The occurrence of palmitate (C16) in the B. bronchiseptica lipid A structures is shown for the first time at two sites. Their presence was also demonstrated for the first time in correlation with the virulence phase of B. bronchiseptica clinical isolates. The recently identified glucosamine modifications of Bordetella lipids A are also reported in these isolates.
structure, lipid A, endotoxin, mass spectrometry, Bordetella bronchiseptica, virulence factor
Structure type: oligomer ; 2148
Location inside paper: p.1080, fig.2A
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141181,IEDB_141807,IEDB_151531,IEDB_176772,IEDB_534864
Methods: mild acid hydrolysis, GC, MALDI-TOF MS, composition analysis, genetic methods
Comments, role: virulent phase isolates
Related record ID(s): 31178, 31179
NCBI Taxonomy refs (TaxIDs): 518
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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