Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Bordetella pertussis [ICD11:
XN23B 
]
The structure was elucidated in this paperNCBI PubMed ID: 21567324Publisher: Totowa, NJ: Humana Press
Editors: Holst O, Walker JM, Beck A
Correspondence: martine.caroff
igmors.u-psud.fr
Institutions: Equipe 'Structure et Activites des Endotoxines', Institut de Genetique et Microbiologie, Universite de Paris Sud-XI, Orsay, France
Lipopolysaccharides (LPSs) are major components of the external membrane of Gram-negative bacteria, and act as an effective permeability barrier. They are essentially composed of a hydrophilic polysaccharide region linked to an hydrophobic one, termed lipid A. Depending on their individual variable fine structures, they may be potent immunomodulators. Because of the structural importance and role of lipid A in bacterial pathogenesis, herein we describe two rapid practical micromethods for structural analysis. The first method allows the direct isolation of lipid A from whole bacteria cell mass; the second describes conditions for the sequential release of fatty acids, enabling the determination of their substitution position in the lipid A structure to be determined by matrix-assisted laser desorption/ionization mass spectrometry. Examples are given with reference to two major pathogens: Bordetella pertussis and Pseudomonas aeruginosa.
Lipopolysaccharide, Lipooligosaccharide, 3-deoxy-D-manno-oct-2-ulosonic acid, tumor necrosis factor, Sodium Dodecyl Sulfate, Gas chromatography, Matrix-assisted laser desorption/ionization mass spectrometry
Structure type: oligomer ; 1559
Location inside paper: p.138, fig.1
Compound class: lipid A
Contained glycoepitopes: IEDB_141806,IEDB_142488,IEDB_144998,IEDB_145002,IEDB_146664,IEDB_176772,IEDB_983931,SB_192
Methods: SDS-PAGE, TLC, MALDI-MS, alkaline treatment, GS-MS
Comments, role: review, methodology
Related record ID(s): 31165, 31166, 31167, 31168, 31169, 31170, 31171
NCBI Taxonomy refs (TaxIDs): 520
Show glycosyltransferases
There is only one chemically distinct structure:
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