CSDB: Search results

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11: XN6P4

]

NCBI PubMed ID: 21372091
Publication DOI: 10.1099/mic.0.046912-0
Journal NLM ID: 0376646
Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
Correspondence: Katarzyna Duda <kduda

fz-borstel.de>
Institutions: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, D-23845 Borstel, Germany, Division of Immunochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, D-23845 Borstel, Germany, Division of Medical and Biochemical Microbiology, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, D-23845 Borstel, Germany, Institute for Molecular Infection Biology, Julius-Maximilians-University of Würzburg, D-97070 Würzburg, Germany, Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University of Göttingen, D-37077 Göttingen, Germany, Institute for Hygiene, University of Münster, D-48149 Münster, Germany

Mastitis represents one of the most significant health problems of dairy herds. The two major causative agents of this disease are Escherichia coli and Staphylococcus aureus. Of the first, its lipopolysaccharide (LPS) is thought to play a prominent role during infection. Here, we report the O-antigen (OPS, O-specific polysaccharide) structure of the LPS from bovine mastitis isolate E. coli 1303. The structure was determined utilizing chemical analyzes, mass spectrometry as well as 1D and 2D NMR spectroscopy methods. The O-repeating unit was characterized as -[→4)-β-D-Quip3NAc-(1→3)-α-L-Fucp2OAc-(1→4)-β-D-Galp-(1→3)-α-D-GalpNAc-(1→]- in which the O-acetyl substitution was non-stoichiometric. The nucleotide sequence of the O-antigen gene cluster of E. coli strain 1303 was also determined. In large parts, this determinant located between the gnd and galF genes comprises a presently unknown DNA sequence with 13 putative open reading frames. The O-antigen of E. coli 1303 was shown to substitute O-7 of the terminal L,D-heptose of the K-12 core oligosaccharide. Interestingly, the non-OPS-substituted core oligosaccharide represented a truncated version of the K-12 outer core, namely terminal L,D-heptose and glucose were missing, however, it possessed a third Kdo residue in the inner core. On the base of structural and genetic data we show that the mastitis isolate E. coli strain 1303 represents a new subtype of serotype O5 and possesses the K-12 core type which is rather uncommon among human and bovine isolates.

Lipopolysaccharide, structure, O-antigen, Escherichia coli, NMR spectroscopy, gene cluster, Staphylococcus aureus, mastitis

Structure type: oligomer
Location inside paper: p.1751, fig.1
Aglycon: lipid A
Compound class: LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130670,IEDB_133751,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_232584,IEDB_983931,SB_192,SB_7

Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, SDS-PAGE, sugar analysis, GLC, ESI-ICR-MS, Western blotting, de-O-acylation with hydrazine, NMR-1D, methanolysis
Biosynthesis and genetic data: genetic data
Comments, role: major core glycoform after deacylation of the K-12 LPS.

Related record ID(s): 26606, 26607, 26608, 26609, 26610
NCBI Taxonomy refs (TaxIDs): 83333
Reference(s) to other database(s): GTC:G37075LG
Show glycosyltransferases

There is only one chemically distinct structure:

O=C(O)[C@@]1(O)C[C@@H](O[C@]2(C(=O)O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O2)[C@@H](O[C@H]2O[C@H]([C@@H](O)CO)[C@@H](OP(=O)(O)O)[C@H](O[C@H]3O[C@H]([C@@H](O)CO[C@H]4O[C@H]([C@@H](O)CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H](OP(=O)(O)O)[C@H](O[C@H]4O[C@H](CO[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@@H](O)[C@H](O[C@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O[C@H]5O[C@H](CO[C@H]6O[C@H]([C@@H](O)CO)[C@@H](O)[C@H](O)[C@@H]6O)[C@@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@@H]([C@H](O)CO)O1

SVG MW SMILES 3D mol Ionize