Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Salmo salar
Associated disease: winter ulcer
The structure was elucidated in this paperNCBI PubMed ID: 22099250Publication DOI: 10.1016/j.carres.2011.10.033Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: L. Kenne <lennart.kenne
slu.se>
Institutions: The Norwegian College of Fishery Science, University of Tromso, NO-9037 Tromso, Norway
The structure of the O-specific side chain of the lipopolysaccharide from the Gram-negative psychrophilic bacterium Moritella viscosa strain M2-226, responsible for the winter ulcer in Atlantic salmon, has been determined. Monosaccharide analysis and (1)H and (13)C NMR spectroscopy were employed to elucidate the structure. It was concluded that the polysaccharide is composed of a trisaccharide repeating unit with the following structure: →3)-β-D-GlcpNAc-(1→4)-[α-D-GlcpA-(1→3)]-α-L-Fucp-(1→
Lipopolysaccharide, O-Polysaccharide structure, Moritella viscosa, winter ulcer, Salmo salar, Atlantic salmon
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.166
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_135866,IEDB_136045,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_142489,IEDB_144562,IEDB_145669,IEDB_150092,IEDB_151531,IEDB_152214,IEDB_174333,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, acid hydrolysis
NCBI Taxonomy refs (TaxIDs): 80854Reference(s) to other database(s): GTC:G30597NU
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,2 Ac 175.1 23.0
4 bDGlcpN 101.7 56.6 80.6 69.0 76.7 61.2
3 aDGlcpA 100.2 72.2 73.3 72.0 72.0 174.2
aLFucp 100.2 68.9 74.8 78.9 68.1 16.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,2 Ac - 1.98
4 bDGlcpN 4.61 3.83 3.65 3.50 3.35 3.74-3.95
3 aDGlcpA 5.29 3.55 3.76 3.55 4.27 -
aLFucp 4.96 3.93 3.96 4.07 4.29 1.16
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,2 Ac 23.0/1.98
4 bDGlcpN 101.7/4.61 56.6/3.83 80.6/3.65 69.0/3.50 76.7/3.35 61.2/3.74-3.95
3 aDGlcpA 100.2/5.29 72.2/3.55 73.3/3.76 72.0/3.55 72.0/4.27
aLFucp 100.2/4.96 68.9/3.93 74.8/3.96 78.9/4.07 68.1/4.29 16.5/1.16
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,2 | Ac |
| 1.98 | |
| 4 | bDGlcpN | 4.61 | 3.83 | 3.65 | 3.50 | 3.35 | 3.74
3.95 |
| 3 | aDGlcpA | 5.29 | 3.55 | 3.76 | 3.55 | 4.27 |
|
| | aLFucp | 4.96 | 3.93 | 3.96 | 4.07 | 4.29 | 1.16 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,2 | Ac | 175.1 | 23.0 | |
| 4 | bDGlcpN | 101.7 | 56.6 | 80.6 | 69.0 | 76.7 | 61.2 |
| 3 | aDGlcpA | 100.2 | 72.2 | 73.3 | 72.0 | 72.0 | 174.2 |
| | aLFucp | 100.2 | 68.9 | 74.8 | 78.9 | 68.1 | 16.5 |
|
There is only one chemically distinct structure:
Found 
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