Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 21280662Journal NLM ID: 0370536Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University , Stockholm, Sweden
Structural determination of N- and O-linked glycans as well as polysaccharides is hampered by the limited spectral dispersion. The computerized approach CASPER, an acronym for computer assisted spectrum evaluation of regular polysaccharides, uses liquid state NMR data to elucidate carbohydrate structure based on agreement with predicted (1)H and (13)C chemical shifts. We here demonstrate developments based on multiple through-bond J-based correlations that significantly enhance the credence to the sequence connectivities proposed in the analysis exemplified by an oligosaccharide and a bacterial polysaccharide. The approach is also suitable for predicting (1)H and (13)C NMR chemical shifts of synthesized oligosaccharides and glycoconjugates, thereby corroborating a proposed structure.
NMR, CASPER, N-linked glycan, O-linked glycan
Structure type: polymer chemical repeating unit
Location inside paper: p.1516, fig.3, 1, S2.2
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142489,IEDB_144562,IEDB_144990,IEDB_147450,IEDB_150767,IEDB_150948,IEDB_151528,IEDB_152214,IEDB_153553,IEDB_167071,IEDB_174333,IEDB_190606,IEDB_241096,IEDB_461711,IEDB_461719,SB_154,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_25,SB_7,SB_8,SB_86,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, NMR-1D, computer analysis with CASPER
NCBI Taxonomy refs (TaxIDs): 1450177Reference(s) to other database(s): GTC:G12611FV
Show glycosyltransferases
There is only one chemically distinct structure:
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