Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: bacteremia [ICD11:
MA15.0 
]
The structure was elucidated in this paperNCBI PubMed ID: 21167477Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M.M. Corsaro <corsaro
unina.it>
Institutions: Dipartimento di Chimica Organica e Biochimica, Universita di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy
Halomonas stevensii is a Gram-negative, pathogenic, moderately halophilic bacterium isolated from the blood of a renal care patient. It optimally grows at 30-35°C at pH 8-9 and at a sea salt concentration ranging from 3.0% to 7.5%. Gram-negative bacterial infections are closely associated with the presence of the lipopolysaccharides (LPSs) on the outer membrane. These molecules consist of three regions covalently linked: the glycolipid (lipid A), the oligosaccharide region (core region), and the O-specific polysaccharide (O-chain, O-antigen). O-antigen seems to play an important role in the colonization step (adherence) and the ability to bypass host defense mechanisms. For this reason the structure elucidation of the O-chain repeating unit is important to improve knowledge about the role of LPS in the host-pathogen interaction. In this paper, we report the complete structure of the O-chain from the LPS of H. stevensii. The bacterial cells were cultivated and LPS was extracted by the PCP (phenol-chloroform-petroleum ether) method. After mild acid hydrolysis, the lipid A was removed by centrifugation and the obtained polysaccharide was analyzed by means of chemical analysis and one- and two-dimensional NMR spectroscopy giving the following structure: (formula: see text).
Lipopolysaccharide, NMR spectroscopy, structure elucidation, Halomonas
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.363
Trivial name: CPS KK207-2
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, SDS-PAGE, sugar analysis, GLC, mild acid hydrolysis, NMR-1D
Comments, role: one of two different chain of the OPS from H. magadiensis; published polymerization frame was shifted for conformity with other records.
NCBI Taxonomy refs (TaxIDs): 1177117,
150024Reference(s) to other database(s): GTC:G22377LP, GlycomeDB:
25388
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3 bDGlcp 105.5 74.5 75.7 80 76 61.2
4 aDGlcp 100.5 73 73.8 70.2 72.7 61.2
bDGalp 104.2 71.9 81.8 76.9 77 61.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3 bDGlcp 4.59 3.18 3.54 3.55 3.46 3.74-3.89
4 aDGlcp 4.84 3.41 3.67 3.43 4.15 3.71-3.78
bDGalp 4.45 3.66 3.78 4.14 3.71 3.73-3.80
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3 bDGlcp 105.5/4.59 74.5/3.18 75.7/3.54 80/3.55 76/3.46 61.2/3.74-3.89
4 aDGlcp 100.5/4.84 73/3.41 73.8/3.67 70.2/3.43 72.7/4.15 61.2/3.71-3.78
bDGalp 104.2/4.45 71.9/3.66 81.8/3.78 76.9/4.14 77/3.71 61.2/3.73-3.80
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3 | bDGlcp | 4.59 | 3.18 | 3.54 | 3.55 | 3.46 | 3.74
3.89 |
| 4 | aDGlcp | 4.84 | 3.41 | 3.67 | 3.43 | 4.15 | 3.71
3.78 |
| | bDGalp | 4.45 | 3.66 | 3.78 | 4.14 | 3.71 | 3.73
3.80 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3 | bDGlcp | 105.5 | 74.5 | 75.7 | 80 | 76 | 61.2 |
| 4 | aDGlcp | 100.5 | 73 | 73.8 | 70.2 | 72.7 | 61.2 |
| | bDGalp | 104.2 | 71.9 | 81.8 | 76.9 | 77 | 61.2 |
|
There is only one chemically distinct structure:
Found 
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