Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: (Actinopterygii)
Associated disease: motile aeromonad septicemia (MAS)
The structure was elucidated in this paperNCBI PubMed ID: 21377659Publication DOI: 10.1016/j.carres.2011.02.003Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: aturska
hektor.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland
The O-specific polysaccharide obtained by mild-acid degradation of lipopolysaccharide of Aeromonas bestiarum P1S was studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy. The sequence of the sugar residues was determined using (1)H,(1)H NOESY and (1)H,(13)C HMBC experiments. The O-specific polysaccharide was found to be a high-molecular-mass polysaccharide composed of tetrasaccharide repeating units of the structure Since small amounts of a terminal Quip3N residue were identified in methylation analysis, it was assumed that the elucidated structure also represented the biological repeating unit of the O-specific polysaccharide.
Lipopolysaccharide, O-specific polysaccharide, 3-hydroxybutyric acid, Aeromonas bestiarum
Structure type: polymer biological repeating unit
Location inside paper: abstract, p.820
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_149136,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, mild acid hydrolysis, ESI-ICR-MS, NMR-1D, SDS-Tricine-PAGE
NCBI Taxonomy refs (TaxIDs): 105751
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,2,3 lR3HOBut 175.19 45.94 65.60 22.87
3,4,2 bDQuip3N 104.78 75.06 56.35 73.87 74.02 17.85
3,4 bDRibf 107.65 84.28 70.28 83.02 63.30
3 bDGalp 105.83 71.47 73.48 76.85 75.16 61.76
2 Ac 175.06 23.16
aDFucpN 96.85 48.42 78.33 72.07 67.50 16.19
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,2,3 lR3HOBut - 2.35-2.48 4.21 1.23
3,4,2 bDQuip3N 4.89 3.48 3.96 3.20 3.59 1.31
3,4 bDRibf 5.62 4.20 4.21 3.96 3.63-3.85
3 bDGalp 4.42 3.47 3.73 3.98 3.69 3.71-3.73
2 Ac - 2.05
aDFucpN 5.53 4.32 3.79 3.97 3.96 1.25
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,2,3 lR3HOBut 45.94/2.35-2.48 65.60/4.21 22.87/1.23
3,4,2 bDQuip3N 104.78/4.89 75.06/3.48 56.35/3.96 73.87/3.20 74.02/3.59 17.85/1.31
3,4 bDRibf 107.65/5.62 84.28/4.20 70.28/4.21 83.02/3.96 63.30/3.63-3.85
3 bDGalp 105.83/4.42 71.47/3.47 73.48/3.73 76.85/3.98 75.16/3.69 61.76/3.71-3.73
2 Ac 23.16/2.05
aDFucpN 96.85/5.53 48.42/4.32 78.33/3.79 72.07/3.97 67.50/3.96 16.19/1.25
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,2,3 | lR3HOBut |
| 2.35
2.48 | 4.21 | 1.23 | |
| 3,4,2 | bDQuip3N | 4.89 | 3.48 | 3.96 | 3.20 | 3.59 | 1.31 |
| 3,4 | bDRibf | 5.62 | 4.20 | 4.21 | 3.96 | 3.63
3.85 | |
| 3 | bDGalp | 4.42 | 3.47 | 3.73 | 3.98 | 3.69 | 3.71
3.73 |
| 2 | Ac |
| 2.05 | |
| | aDFucpN | 5.53 | 4.32 | 3.79 | 3.97 | 3.96 | 1.25 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,2,3 | lR3HOBut | 175.19 | 45.94 | 65.60 | 22.87 | |
| 3,4,2 | bDQuip3N | 104.78 | 75.06 | 56.35 | 73.87 | 74.02 | 17.85 |
| 3,4 | bDRibf | 107.65 | 84.28 | 70.28 | 83.02 | 63.30 | |
| 3 | bDGalp | 105.83 | 71.47 | 73.48 | 76.85 | 75.16 | 61.76 |
| 2 | Ac | 175.06 | 23.16 | |
| | aDFucpN | 96.85 | 48.42 | 78.33 | 72.07 | 67.50 | 16.19 |
|
There is only one chemically distinct structure:
Found 
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